Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (46): 8617-8620.doi: 10.3969/j.issn.1673-8225.2011.46.016

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Effects of magnetic stimulation on nerve conduction velocity and the expression of growth associated protein 43 in rats after sciatic nerve injury

Wang Wei1, Yuan Xiu-hua2, Wang Zhong-li2, Liu Ning2, Li Long-guang1   

  1. 1Department of Rehabilitation, Third Affiliated Hospital of Liaoning Medical College, Jinzhou 121000, Liaoning Province, China;
    2Department of Rehabilitation, First Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
  • Received:2011-04-19 Revised:2011-08-16 Online:2011-11-12 Published:2011-11-12
  • About author:Wang Wei★, Master, Associate chief physician, Department of Rehabilitation, Third Affiliated Hospital of Liaoning Medical College, Jinzhou 121000, Liaoning Province, China dubutianxia315@163.com
  • Supported by:

    the Foundation of Liaoning Educational Committee, No. 20061007* 

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Abstract:

BACKGROUND: Magnetic stimulation may promote the repair of nerve injury.
OBJECTIVE: To explore the effect of magnetic stimulation on nerve conduction velocity and growth associated protein 43 expression of corresponding spinal motor neuron in rats after sciatic nerve injury.
METHODS: A number of 60 SD rats were randomly assigned into 3 groups: experiment group (n=24), model group (n=24) and sham-operation group (n=12). The mouse sciatic nerve was clamped by a new hemostatic forcep with a length of 17 centimeters. The clamping tension was adjusted by locking the 2nd interlocking teeth with a pressure of 21.95×103 Pa, lasting for 10 seconds to construct sciatic nerve injury model. At 24 hours after the model construction, the experiment mice were treated with a daily magnetic stimulation of 0.09 T.
RESULTS AND CONCLUSION: According to immunohistochemical staining, at the 2nd, 4th, 8th and 12th weeks after model construction, the expression of growth associated protein 43 in L4-5 motor neurons in the experiment group was significantly higher than that of the model group at the same time point (P < 0. 05). Electrophysiology examination showed that compare with the model group, the regenerated nerve of the experiment group had faster conduction velocity, higher amplitude and shorter latent period at the 12th week after model construction (P < 0.05). These results demonstrate that the magnetic stimulation can speed up the nerve conduction of injured sciatic nerve, increase the expression of growth associated protein 43 in corresponding motor neurons and promote the repair of sciatic nerve injury in rats.

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