Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (46): 8613-8616.doi: 10.3969/j.issn.1673-8225.2011.46.015

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A mouse model of lupus induced by nucleoprotein with the same genetic background

Xie Jun, Lin You-kun   

  1. Department of Dermatovenereology, First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-04-02 Revised:2011-04-12 Online:2011-11-12 Published:2011-11-12
  • Contact: Lin You-kun, Master’s supervisor, Chief physician, Professor, Department of Dermatovenereology, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China linyoukun7@yahoo.com.cn
  • About author:Xie Jun★, Master, Physician, Department of Dermatovenereology, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China xiejunz@sina.com
  • Supported by:

    Youth Science Foundation of Guangxi Zhuang Autonomous Region, No. guikeqing0542055*; A Key Science and Technology Program of Guangxi Department of Health, No. Guiweizhong 200829*

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Abstract:

BACKGROUND: A mouse model of spontaneous lupus cannot be used for study of pathogenic factors other than genetic factors.
OBJECTIVE: To establish a mouse model of lupus induced by Balb/c mouse nucleoprotein with the same genetic background.
METHODS: Thirty Balb/c mice of SPF grade, aged 4-6 weeks, were randomly and evenly divided into three groups. The V1 group mice were intramuscularly administered nucleoprotein extracted from the same strains of Balb/c mice, once every other 3 weeks, for a total of four times. The V2 group mice were administered the same volume of phosphate buffered saline. The V3 group mice served as normal controls. The 24-hour urine protein, serum antinuclear antibody, anti-ds-DNA antibody and direct immunofluorescence of mouse kidney were detected at 3 weeks after the last immunization.
RESULTS AND CONCLUSION: The 24-hour urine protein, anti-ds-DNA antibody, and antinuclear antibody in the V1 group were significantly higher than those in the V2 and V3 groups. There was IgG immune complex deposition in the renal glomerulus of V1 group. However, in the V2 and V3 groups, renal glomerulus contour was not observed, and only non-specific fluorescence could be observed. A mouse model of lupus can be successfully induced by immunizing Balb/c mouse with Balb/c mouse nucleoprotein with the same genetic background.

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