Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (46): 8551-8555.doi: 10.3969/j.issn.1673-8225.2011.46.001

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Effects of serum on tumor necrosis factor alpha induced chondrocyte apoptosis following electro-acupuncture

Wu Ming-xia1, Li Xi-hai2, Wu Guang-wen2, Li Li1, Chen Wen-lie3, Liu Xian-xiang2   

  1. 1Department of Acupuncture and Moxibustion, the Second Affiliated People’s Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China
    2Fujian Academy of Integrative Medicine, Fuzhou  350108, Fujian Province, China
    3Fujian Senile Disease of Integrative Medicine Key Laboratories, Fuzhou 350108, Fujian Province, China
  • Received:2011-03-19 Revised:2011-06-12 Online:2011-11-12 Published:2011-11-12
  • Contact: Liu Xian-xiang, Doctor, Professor, Fujian Academy of Integrative Medicine, Fuzhou 350108, Fujian Province, China liuxianxiang@163. com
  • About author:Wu Ming-xia☆, Doctor, Chief physician, Professor, Department of Acupuncture and Moxibustion, the Second Affiliated People’s Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China wumingxiafz@163. com
  • Supported by:

    the National Natural Science Foundation of China, No. 30873277*; Chen Ke-ji Integrative Medicine Development Foundation, No. CKJ2008003*

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Abstract:

BACKGROUND: Electro-acupuncture (EA) can promote blood circulation, remove blood stasis, regulate vital energy circulation to relieve pain, relieve rigidity of muscles and activate collaterals, and effectively relieve symptoms of osteoarthritis.
OBJECTIVE: To observe the effect of serum on tumor necrosis factor α (TNF-α) induced chontroctye apoptosis following EA.
METHODS: Knee joint chondrocytes from 3-week-old SD rats were isolated using enzymatic digestion and apoptosis induced with 10 μg/L TNF-α in vitro. The normal group rats were routinely raised and did not receive any treatment. The osteoarthritis rats were routinely raised, intraarticularly injected with sodium hyaluronate (0.4 μL/g, once a week, for successive 5 weeks), or given EA at the Neixiyan and Waixiyan ( 5, 15 and 30 min/d), and all rats were interfered with serum, and then the apoptotic rate of the chondrocytes was determined.
RESULTS AND CONCLUSION: After 15 and 30 minutes of EA at the Neixiyan and Waixiyan, osteoarthritis rats were interfered with serum for 24 hours, the activity of chondrocytes was significantly increased and the apoptotic rate of the chondrocytes was significantly decreased. These findings suggest that serum can inhibit TNF-α induced chondrocyte apoptosis following EA.

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