Abstract:

BACKGROUND: The SDF-1/CXCR4 axis system is consisted of CXCR4 and stromal cell-derived factor -1 (SDF-1). The SDF-1/CXCR4 axis system plays an important role in regeneration and repair of tissue and bone injury.
OBJECTIVE: To investigate expression and significance of CXCR4 in rat bone tissue around implants during early implantation.
METHODS: Twenty Wistar rats of 4 months old were selected and randomly divided into implantation 6-, 12- and 24-hour groups and blank control group with 5 mice in each group. All rats except blank control group received anodically oxidized implants in the left tibia. Rats were killed 6, 12, 24 hours after implantation, the implants were unscrewed, and bone tissues around implants were prepared, 2 cm in diameter. For blank control group, 2 cm proximal epiphysis bone tissues were prepared. Then protein samples were then prepared.
RESULTS AND CONCLUSION: a large number of fibers could be seen on the anodized implant surface in each group. the grey values of CXCR4/β-actin were significantly higher in the implantation 6-, 12- and 24-hour groups than the blank control group (P > 0.01). The expression of CXCR4 during the first 24 hours of implantation was obviously higher than that of the blank control group, indicating that CXCR4 positive cells take part in the regeneration of bone tissue during early osseointegration.