Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (38): 7053-7056.doi: 10.3969/j.issn.1673-8225.2011.38.006

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Effect of fibrin glue associated with antisense to PCNA on preventing restenosis of vein grafts

Wan Li, Wang Wen-jun, Cao Yuan-ping, Wang Qun, Liu Ji-chun   

  1. Department of Cardiothoracic Surgery, First Affiliated Hospital, Nanchang University, Nanchang  330006, Jiangxi Province, China
  • Received:2011-03-24 Revised:2011-06-02 Online:2011-09-17 Published:2011-09-17
  • Contact: Liu Ji-chun, Professor, Doctoral supervisor, Chief physician, Department of Cardiothoracic Surgery, First Affiliated Hospital, Nanchang University, Nanchang 330006, Jiangxi Province, China Liujichun999@yahoo.com.cn
  • About author:Wan Li☆, Doctor, Associate professor, Master’s supervisor, Associate chief physician, Department of Cardiothoracic Surgery, First Affiliated Hospital, Nanchang University, Nanchang 330006, Jiangxi Province, China wlzh7580@yahoo. com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30760247*, 30960380*

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Abstract:

BACKGROUND: Preliminary findings show that fibrin glue is not only a good non-restrictive, extravascular biodegradable stent, but also can prevent intimal and medial hyperplasia of vein grafts. It is also a good drug delivery system that can improve the extravascular membrane gene transfection efficiency.
OBJECTIVE: To verify the effect of fibrin glue associated with antisense to PCNA on preventing restenosis of vein grafts.
METHODS: Rabbit models of external jugular vein carotid artery bypass grafting were prepared and then randomized into model group, fibrin glue group and fibrin glue+antisense group. Commercially available fibrin glue and fibrin glue mixed with adenovirus expressing the antisense oligocleotides to PCNA were applied separately around vein grafts in the latter two groups, respectively.
RESULTS AND CONCLUSION: Twenty-eight days after operation, the intimal and medial thickness and area was increased obviously in the model group and decreased significantly in the fibrin glue group (P < 0.01). A significant difference in the intimal and medial thickness and area was found between the fibrin glue group and fibrin glue+antisense group (P < 0.05). The mRNA and protein expressions of PCNA in the fibrin glue+antisense group was lower than those in the fibrin glue group (P < 0.05). The expression of PCNA in vein grafts can be inhibited by adventitial delivery of antisense to PCNA. The fibrin glue mixed with antisense has a synergistic effect on reducing the intimal and medial thickness and area of vein grafts.

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