Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (12): 2113-2116.doi: 10.3969/j.issn.1673-8225.2011.12.006

Previous Articles     Next Articles

Nano-hydroxyapatite/bone morphogenetic protein compound for repair of segmental radius bone defects of rabbits and local vascular endothelial growth factor expression

Chang Qi1, Huang Chang-lin2, Huang Tao1   

  1. 1Department of Orthopaedics, the 150th Central Hospital of Chinese PLA, Luoyang  471031, Henan Province, China; 2Institute of Military Medicine of PLA, the 150th Central Hospital of Chinese PLA, Luoyang  471031, Henan Province, China
  • Received:2010-10-28 Revised:2011-01-15 Online:2011-03-19 Published:2011-03-19
  • About author:Chang Qi☆, Doctor, Associate chief physician, Department of Orthopaedics, the 150th Central Hospital of Chinese PLA, Luoyang 471031, Henan Province, China changqi1127@yahoo.com.cn
  • Supported by:

    Medical and Health Scientific Research Project of Chinese PLA, No. 06Q024*

PDF

350

Abstract:

BACKGROUND: Previous studies demonstrated that bone or biomimetic bone tissue is implanted into a cantlet of bone defect, necrotic tissue is gradually replaced, the active vascular granulation tissue grow in bone graft, the bone graft is absorbed, and new bone is formed initiatively. However, the process occurs slowly and incomplete in segmental bone defect.
OBJECTIVE: To observe the ability of repairing segmental bone defect and induced vasculogenesis with Nano-hydroxyapatite-collagen (NHAC) materials combined with bone morphogenetic protein (BMP).
METHODS: Segmental radius bone defect models were prepared, which were randomly divided into 2 groups by sortition, NHAC/BMP and NHAC was implanted into one side of models to repairing bone defect, respectively, the other side as blank control group. The effects were observed by gross observation, X-ray examination, histomorphological examination, analysis of histological section alkaline phosphatase staining and bone formation content, positive cell rate and the number of positive vessels of vascular endothelial growth factor (VEGF) detection.
RESULTS AND CONCLUSION: There was almost no bone tissue formation in blank control group. NHAC was divided into nubble after implantation; the original structure of materials was damaged. The remaining materials in NHAC/BMP group were less than that in NHAC group, the degradation of materials was more thorough. Bone formation content, VEGF expression, and the number of positive vessels of VEGF of NHAC/BMP in blank control group were higher than that in NHAC group (P < 0.001). VEGF expression was proportional relationship with the number of positive vessels of VEGF. After NHAC combined with BMP, the repair capacity of bone was further reinforced, and the ability of induced angiogenesis was significantly improved.

CLC Number: