Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (42): 6324-6329.doi: 10.3969/j.issn.2095-4344.2016.42.014

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Neuron-secreted factors promote the growth of skeletal muscle cells

Cai Ming-xuan1, Wang Mei-chen1, Xu Zhi-cheng1, Yan Nan2, Meng Xin-yu1, Wang Zheng-dong3   

  1. 1Basic Medical School, 2Department of Rehabilitation, 3Department of Anatomy, Shenyang Medical University, Shenyang 110034, Liaoning Province, China
  • Received:2016-08-30 Online:2016-10-14 Published:2016-10-14
  • Contact: Wang Zheng-dong, M.D., Department of Anatomy, Shenyang Medical University, Shenyang 110034, Liaoning Province, China
  • About author:Cai Ming-xuan, Basic Medical School, Shenyang Medical University, Shenyang 110034, Liaoning Province, China
  • Supported by:

    the Innovative Training Program for College Students of Liaoning Province, No. 20151161; the College Students Scientific Research Project of Shenyang Medical University, No. 20150801

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Abstract:

BACKGROUND: There is no effective treatment for muscle atrophy caused by peripheral nerve damage. Skeletal muscle cells, a structural unit of muscle contraction, can be used for studies on muscle atrophy when cultured in vitro.
OBJECTIVE: To investigate the promotion effect of neuron-secreted factors on the growth of skeletal muscle cells in vitro.
METHODS: Skeletal muscle cells primary cultured in vitro were divided into two groups: experimental group with neuron-secreted factors, and control group with common culture medium, respectively. Afterwards, the number of skeletal muscle cells and expression level of alpha actin were detected by hematoxylin-eosin staining and immunohistochemical staining, respectively.
RESULTS AND CONCLUSION: The number of skeletal muscle cells and expression level of alpha actin in the experimental group were significantly higher than those in the control group (P < 0.05). In conclusion, neuron-secreted factors have the ability of promoting the growth of skeletal muscle cells and may be helpful for denervated muscle atrophy.
Subject headings: Tissue Engineering; Satellite Cells, Skeletal Muscle; Actinin; Neurons; Cells, Cultured

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

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