Caffeic acid phenethyl ester against cellular injuries in the rotenone-induced Parkinson’s disease model

doi:10.3969/j.issn.2095-4344.2016.40.008

Abstract

Abstract:

BACKGROUND: Caffeic acid phenethyl ester (CAPE) can inhibit lipid peroxidation after rat brain injury. However, the trend of 5-lipoxygenaseis (5-LOX) and cysteinyl leukotrienes (CysLTs) in model of Parkinson’s disease, and whether CAPE protects against rotenone-induced cellular injuries by inhibiting the levels of 5-LOX and CysLTs still need further research.

OBJECTIVE: To investigate the protective effect of CAPE on the rotenone-induced Parkinson-like injury, and to determine whether 5-LOX involved.

METHODS: (1) PC12 cells in good-growth were collected and divided into five groups cultured with different concentrations of rotenone (0, 0.01, 0.1, 1, 10 μmol/L). 24 and 48 hours later, changes of cellular morphology and activity were observed to single out the optimum concentration of rotenone; at 24 hours, the levels of 5-LOX and CysLTs were detected by western blotting and ELISA, respectively. (2) PC12 cells were pretreated with different concentrations of CAPE (0, 0.01, 0.1, 1, 10 µmol/L) for 30 minutes, and 1 µmol/L rotenone was then added. The other cells received no intervention as blank control group. Subsequently, the cell activity was detected, and the CysLTs production was detected by ELISA at 24 hours.

RESULTS AND CONCLUSION: (1) Rotenone (0.1-10 μmol/L) could induce PC12 cell injury with overt morphological and cell activity changes at 24 hours, especially the 1 µmol/L rotenone. (2) Rotenone also significantly increased the 5-LOX expression and CysLTs production in a concentration-dependant manner. (3) CAPE (1-10 μmo/L) significantly attenuated rotenone-induced CysLTs production and cell viability reduction in a concentration-dependant manner. (4) These results suggest that CAPE protects against PC12 cell injuries in the model rat with Parkinson’s disease induced by rotenone involving 5-Lox.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Rotenone, Parkinson Disease, PC12 Cells, Tissue Engineering

CLC Number:

R318

Qiu Shi1, Li Jun-guo1, Qiu Qian2, Chen Hui1, Xiang Zi-min3. Caffeic acid phenethyl ester against cellular injuries in the rotenone-induced Parkinson’s disease model[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(40): 5979-5985.

Figures/Tables 5

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