Endothelial-like cells versus human umbilical vein endothelial cells

doi:10.3969/j.issn.2095-4344.2016.01.015

Abstract

Abstract:

BACKGROUND: Stem cells are induced to differentiate into endothelial-like cells that can be used for the treatment of diabetic lower extremity vascular disease. However, it is unclear whether these endothelial-like cells can completely replace endothelial cells to improve vascular disease and what are the differences between endothelial-like cells and endothelial cells.
OBJECTIVE: To explore the differences and similarities between endothelial-like cells and human umbilical vein endothelial cells in the aspects of morphology, function, and viability.
METHODS: Umbilical cord mesenchymal stem cells and umbilical vein endothelial cells were isolated, cultured and identified using flow cytometry and immunohistochemical method. Isolated umbilical cord mesenchymal stem cells were induced in DMEM-LG/F12 containing 10 µg/L vascular endothelial growth factor, 10 µg/L basic
fibroblast growth factor and 2% fetal bovine serum to differentiate into endothelial-like cells followed by immunohistochemical identification. To compare endothelial-like cells with human umbilical vein endothelial cells, cell migration detection, active substance measurement and three-dimensional angiogenesis test were performed.
RESULTS AND CONCLUSION: Isolated umbilical cord mesenchymal stem cells strongly expressed the surface markers of mesenchymal stem cells, and human umbilical vein endothelial cells strongly expressed CD31 and VWF. After induction, the umbilical cord mesenchymal stem cells were identified to highly express CD31 and VWF. Through cell migration, active substance and three-dimensional angiogenesis tests, endothelial-like cells were similar to endothelial cells in the function and activity, and superior to endothelial cells.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Hao Xiao-juan, Hao Hai-ying, Zhu Min-jie, Yuan Zheng, Li Wei-wei, Chen Jie, Zhu Lv-yun. Endothelial-like cells versus human umbilical vein endothelial cells [J]. Chinese Journal of Tissue Engineering Research, 2016, 20(1): 83-88.

Figures/Tables 7

References

[1] Barry F, Murphy M. Mesenchymal stem cells in joint disease and repair.Nat Rev Rheumatol. 2013;9(10):584-594.

[2] Qi Y, Feng G, Yan W. Mesenchymal stem cell-based treatment for cartilage defects in osteoarthritis. Mol Biol Rep. 2012;39(5):5683-5689.

[3] Heydarkhan-Hagvall S, Schenke-Layland K, Yang JQ, et al. Human adipose stem cells: a potential cell source for cardiovascular tissue engineering. Cells Tissues Organs. 2008;187(4):263-274.

[4] Hynes K, Menicanin D, Han J, et al. Mesenchymal stem cells from iPS cells facilitate periodontal regeneration. J Dent Res. 2013;92(9):833-839.

[5] Nekanti U, Rao VB, Bahirvani AG, et al. Long-term expansion and pluripotent marker array analysis of Wharton's jelly-derived mesenchymal stem cells. Stem Cells Dev. 2010;19(1):117-130.

[6] Wexler SA, Donaldson C, Denning-Kendall P, et al. Adult bone marrow is a rich source of human mesenchymal 'stem' cells but umbilical cord and mobilized adult blood are not. Br J Haematol. 2003;121(2):368-374.

[7] Secco M, Zucconi E, Vieira NM, et al. Multipotent stem cells from umbilical cord: cord is richer than blood. Stem Cells. 2008;26(1):146-150.

[8] Chen MY, Lie PC, Li ZL, et al. Endothelial differentiation of Wharton's jelly-derived mesenchymal stem cells in comparison with bone marrow-derived mesenchymal stem cells. Exp Hematol. 2009;37(5):629-640.

[9] Wu R, Tang Y, Zang W, et al. MicroRNA-128 regulates the differentiation of rat bone mesenchymal stem cells into neuron-like cells by Wnt signaling. Mol Cell Biochem. 2014;387(1-2):151-158.

[10] Ohgushi H. Osteogenically differentiated mesenchymal stem cells and ceramics for bone tissue engineering. Expert Opin Biol Ther. 2014;14(2):197-208.

[11] Panfilov IA, de Jong R, Takashima S, et al. Clinical study using adipose-derived mesenchymal-like stem cells in acute myocardial infarction and heart failure. Methods Mol Biol. 2013;1036:207-212.

[12] Bhonde RR, Sheshadri P, Sharma S, et al. Making surrogate β-cells from mesenchymal stromal cells: perspectives and future endeavors. Int J Biochem Cell Biol. 2014;46:90-102.

[13] 田新,符仁义,陈艳,等脐带间充质干细胞分离及向成骨与脂肪细胞的分化[J].四川大学学报,2008,39(1):26-29.

[14] Zhang X, Nan Y, Wang H, et al. Model microgravity enhances endothelium differentiation of mesenchymal stem cells. Naturwissenschaften. 2013;100(2):125-133.

[15] Guan XM, Cheng M, Li H, et al. Biological properties of bone marrow-derived early and late endothelial progenitor cells in different culture media. Mol Med Rep. 2013;8(6):1722-1728.

[16] Lai WH, Ho JC, Chan YC, et al. Attenuation of hind-limb ischemia in mice with endothelial-like cells derived from different sources of human stem cells. PLoS One. 2013;8(3): e57876.

[17] Portalska KJ, Chamberlain MD, Lo C, et al. Collagen modules for in situ delivery of mesenchymal stromal cell-derived endothelial cells for improved angiogenesis. J Tissue Eng Regen Med. 2013 Apr 17. [Epub ahead of print]

[18] Vishnubalaji R, Manikandan M, Al-Nbaheen M, et al. In vitro differentiation of human skin-derived multipotent stromal cells into putative endothelial-like cells. BMC Dev Biol. 2012;12:7.

[19] Marchal JA, Picón M, Perán M, et al. Purification and long-term expansion of multipotent endothelial-like cells with potential cardiovascular regeneration. Stem Cells Dev. 2012; 21(4):562-574.

[20] Yan D, Wang X, Li D, et al. Macrophages overexpressing VEGF, transdifferentiate into endothelial-like cells in vitro and in vivo. Biotechnol Lett. 2011;33(9):1751-1758.

[21] Marchand M, Anderson EK, Phadnis SM, et al. Concurrent generation of functional smooth muscle and endothelial cells via a vascular progenitor. Stem Cells Transl Med. 2014;3(1): 91-97.

[22] Choi M, Lee HS, Naidansaren P, et al. Proangiogenic features of Wharton's jelly-derived mesenchymal stromal/stem cells and their ability to form functional vessels. Int J Biochem Cell Biol. 2013;45(3):560-570.

[23] 刘红,俞小芳,滕杰,等.低氧预处理对大鼠骨髓间充质干细胞迁移能力的影响[J].中华医学杂志,2012,92(10):703-719.

[24] 朱斌,王云雅,张志勇,等.大鼠内皮祖细胞的分离、培养与定向诱导分化[J].现代中西医结合杂志,2009,18(6):608-611.

[25] 邵琴,王长谦,范华骅,等.脐血、外周血内皮祖细胞分化为内皮细胞的实验研究[J].心脏杂志,2006,18(1):18-22.