Biocompatibility of double-layer poly(L-lactic acid) electrospun nanofiber scaffold with human periodontal ligament cells

doi:10.3969/j.issn.2095-4344.2015.52.014

Abstract

Abstract:

BACKGROUND: The morphological structure of nanofiber scaffold which fabricated by electrospinning technique is similar to the natural extracellular matrix, which provides a good microenvironment for cell growth and proliferation, and can also enhance cell adhesion, migration, proliferation and differentiation.
OBJECTIVE: To observe the biocompatibility of double-layer poly(L-lactic acid) electrospun nanofiber scaffold and human periodontal ligament cells.
METHODS: Electrospinning technique was used to prepare double layers poly(L-lactic acid) electrospun nanofiber scaffold. The toxicity of different concentrations of (100%, 75%, 50%, 25%) double-layer poly(L-lactic acid) electrospun nanofiber scaffold extracts to human periodontal ligament cells was evaluated by MTT assay. The double-layer poly(L-lactic acid) electrospun nanofiber scaffold was co-cultured with human periodontal ligament cells. The cell adhesive capacity in early stage was determined by MTT assay. The growth of cells on the scaffold was observed by scanning electron microscopy.
RESULTS AND CONCLUSION: Different concentrations of double-layer poly(L-lactic acid) electrospun nanofiber scaffold extracts did not create any toxicity to human periodontal ligament cells. After co-culture for 2, 6, 24 hours,
human periodontal ligament cells were poorly adherent onto the double-layer poly(L-lactic acid) electrospun nanofiber scaffold. After 7 days of co-culture, human periodontal ligament cells adhered well on the loose surface of scaffold, maintained the original shape, stretched well, and interconnected processes were observed; on the dense surface of the scaffold, multi-layer cells were observed. The cells showed fusiform or polygonal appearance and were connected together. These results demonstrate that the double-layer poly(L-lactic acid) electrospun nanofiber scaffold has good biocompatibility with human periodontal ligament cells.

中国组织工程研究杂志出版内容重点：人工关节；骨植入物；脊柱；骨折；内固定；数字化骨科；组织工程

CLC Number:

R318

Sun Wen-juan, Jiang Hao-shun, Huang Nan-nan, Tang Qian, Yang Yu-hong. Biocompatibility of double-layer poly(L-lactic acid) electrospun nanofiber scaffold with human periodontal ligament cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(52): 8438-8483.

Figures/Tables 6

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