Silk fibroins from different sources repair osteochondral defects

doi:10.3969/j.issn.2095-4344.2015.52.009

Abstract

Abstract:

BACKGROUND: At present, there are no studies of comparing the effect of silk fibroins from different sources in repair of osteochondral defects.
OBJECTIVE: To compare the effect of mulberry silk- and tussah-derived silk fibroin scaffold materials in repair of osteochondral defect.
METHODS: Totally 20 New Zealand white rabbits were obtained to prepare osteochondral defect models on the unilateral knee joint and randomly divided into five groups: control group, experimental group 1, experimental group 2, experimental group 3 and experimental group 4. Rabbits in the control group were not implanted any materials. In the experimental group 1, 3 layers of mulberry silk protein scaffolds stuck together to fill in defects. In the experimental group 2, one mulberry silk protein scaffold coated with transforming growth factor-β3 was stuck with two mulberry silk protein scaffolds coated with bone morphogenetic protein-2 to fill in defects. In the experimental group 3, three layers of tussah protein scaffolds stuck together to fill in defects. In the experimental group 4, one tussah protein scaffold coated with transforming growth factor-β3 stuck together with two tussah protein scaffolds coated with bone morphogenetic protein-2 to fill in defects. At 8 weeks post surgery,
articular cartilage repair area was observed histopathologically. Type I and II collagen expressions were determined.
RESULTS AND CONCLUSION: The collagen fibers in experimental group 1 were widely distributed in the full-thickness defect area. The collagen fibers in the experimental group 2 were parallelly distributed on the surface of repair area, vertically distributed from the middle and bottom to the top direction. Collagen was observed on the surface of repair area in the experimental group 3. The cartilage-like cells presented clumped distribution on the surface and at the bottom of scaffold. The type I collagen expression in the repair area was strongly positive in these four experimental groups. The type II collagen expression in the repair area of experimental 1 and experimental 2 groups was weak. The type II collagen expression in the repair area of experimental 3 and experimental 4 groups was strongly positive. These results demonstrate that these two kinds of silk fibroins can both repair osteochondral defects, in which mulberry silk proteins tend to form bone tissue, and tussah silk proteins tend to form cartilage tissue.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

CLC Number:

R318

Wang Qian, Ma Yun-sheng, Li De-hua. Silk fibroins from different sources repair osteochondral defects[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2015.52.009.

Figures/Tables 2

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