Inhibitory effect of Sema7A siRNA on osteoclast activation induced by titanium particles

doi:10.3969/j.issn.2095-4344.2015.52.004

Abstract

Abstract:

BACKGROUND: Sema7A is a kind of cell surface protein, which can promote the fusion of osteoclasts and the migration of osteoblast at the same time, affecting the dynamic balance of bone.
OBJECTIVE: To investigate whether Sema7A siRNA has ainhibitory effect on the osteoclast activation in the process of osteolysis which induced by titanium particles.
METHODS:The precursor osteoclasts with the concentration of 4×109/L were seeded on 96-well plates containing glass cover slips, and divided into four groups: blank control, positive control, experiment and negative control groups. The cell culture medium was added into the control group. 20 μL un-transfected siRNA supernatant was added into the positive control group. 20 μL transfected Sema7A siRNA supernatant was added into the experiment group. 20 μL transfected control siRNA supernatant was added into the negative control group. The supernatant was obtained through the co-culture between titanium particles solution and monocyte-macrophage cell line RAW264.7of mouse for 24 hours. siRNA was transfected into mononuclear macrophage cell lines RAW264.7 of mice.
RESULTS AND CONCLUSION: At 7 days of culture, the expression levels of interleukin-1, interleukin-1β, tumor necrosis factor α, matrix metalloproteinase-9 and the receptor activator of nuclear factor-κB in the positive control,
negative control and experiment groups were higher than those in the control group (P < 0.05). The expression level of each factor in the experiment group was lower than that in the positive control and negative control groups (P < 0.05). At 8 days of culture, the proliferation activity of osteoclasts and the number of positive cells stained by tartrate-resistant acid phosphatase in the positive control, negative control and experiment groups were higher than those in the control group (P < 0.05). The proliferation activity of osteoclasts and the number of positive cells stained by tartrate-resistant acid phosphatase in the experiment group were lower than those in the control and negative groups (P < 0.05). These results demonstrate that Sema7A siRNA has a certain inhibitory effect on the osteoclast activation induced by titanium particles.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

CLC Number:

R318

Cong Yu, Ru Jiang-ying, Zhao Yun-long, Yu Lei, Bao Ni-rong, Xu Bin, Zhao Jian-ning. Inhibitory effect of Sema7A siRNA on osteoclast activation induced by titanium particles[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(52): 8384-8390.

Figures/Tables 6

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