Bone morphogenetic protein complex combined with CAD/CAM technique in the in situ repair of laryngeal cartilage defects

doi:10.3969/j.issn.2095-4344.2015.51.006

Abstract

Abstract:

BACKGROUND: In the process of repairing laryngeal cartilage defects, a certain scaffold material can be used. Bone morphogenetic proteins have the activity of osteogenic induction, which can be used in combination with other materials. In order to improve the repairing effect, the combination of bone morphogenetic protein complex and computer aided design/computer aided manufacturing (CAD/CAM) technology is preferred.
OBJECTIVE: To investigate the effect of bone morphogenetic protein complex combined with CAD/CAM technology for in situ repair of laryngeal cartilage defects.
METHODS: Eighteen New Zealand white rabbits were enrolled and randomly divided into observation and control
groups followed by three-dimensional scanning of the throat. Then, three-dimensional rabbit models of the thyroid cartilage were made. Porous hydroxyapatite scaffold was prepared using gelcasting-foam method and combined with bone morphogenetic proteins. After unilateral removal of the thyroid cartilage, the composite scaffold and gelatin sponge were implanted into the rabbits in the observation and control groups, respectively. At 4, 8, 12 weeks after implantation, the rabbits in the two groups were observed grossly. At 12 weeks, the animals were sacrificed following laryngoscopy to observe the histological and pathological changes.
RESULTS AND CONCLUSION: At 12 weeks after implantation, the laryngeal cavity was narrowed and appeared to have a slight congestion in the control group; while in the observation group, the laryngeal cavity was smooth, unobstructed and spacious, with no granulation growth. At 4, 8, 12 weeks after implantation, the scores on the thyroid cartilage were decreased dramatically in the two groups, especially in the observation group (P < 0.05). At 12 weeks after implantation, there were no mature osteocytes and chondrocytes in the control group; in the observation group, the scaffold degraded completely, there was a large amount of cell proliferation and phagocytosis, and chondroblastic proliferation and cartilage formation were found in the surgical region. These results indicate that the use of bone morphogenetic protein complex combined with CAD/CAM technique can have good achievements in the treatment of in situ laryngeal cartilage defects. Additionally, the composite material used in the experimental procedure has good osteoinductive effect and histocompatibility.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

CLC Number:

R318

Liu Yi-chang, Zhou Jing. Bone morphogenetic protein complex combined with CAD/CAM technique in the in situ repair of laryngeal cartilage defects[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2015.51.006.

Figures/Tables 4

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