Effect of estradiol and progesterone on the number of macrophages and the expression of related factor in the endometrium of ovariectomized mice

doi:10.3969/j.issn.2095-4344.2015.49.022

Abstract

Abstract:

BACKGROUND: Studies have shown that macrophage function and macrophage colony-stimulating factor expression may be directly or indirectly affected by regulation of estrogen and progestational hormone, but the specific impact has not been studied in depth.
OBJECTIVE: To observe the effect of estradiol and progesterone on the number of macrophages and the expression of macrophage colony-stimulating factor in the endometrium of bilaterally ovariectomized mice.
METHODS: The bilaterally ovariectomized mice were randomly divided into estrogen, progestational hormone, and control groups. The number of macrophages and the expression of macrophage colony-stimulating factor in the endometrium of mice in each group were analyzed.
RESULTS AND CONCLUSION: Immunohistochemical staining results showed that after 6 and 24 hours of intervention, the number of macrophages and the expression of macrophage colony-stimulating factor in the mouse endometrium in the estrogen and progestational hormone groups were significantly higher than those in the control group (P < 0.05). These results confirm that both estradiol and progesterone can increase the number of macrophages, and can increase the expression of macrophage colony-stimulating factors in the endometrium of bilaterally ovariectomized mice.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

CLC Number:

R318

Zhao Ying-jian, Han Jun-yan.

Effect of estradiol and progesterone on the number of macrophages and the expression of related factor in the endometrium of ovariectomized mice

[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(49): 8000-8004.

Figures/Tables 3

References

[1] 刘卓,金英,隋海娟,等.知母皂苷对Aβ25-35引起的巨噬细胞炎症介质释放的抑制作用及信号转导机制[J].中国药理学通报, 2011, 27(5):695-700.

[2] 贾永芳,吴雷振,张瑞平,等.花旗松素,槲皮素对 LPS 诱导流产小鼠子宫巨噬细胞的抑制作用[J].中国免疫学杂志, 2013,29(7): 723-727.

[3] 谭洪川.雌,孕激素对小鼠子宫巨噬细胞数量分布的影响及机制的初步探讨[D].广州:南方医科大学,2012.

[4] 冯桂梅,肖丽,黄薇,等.宫腔镜下子宫中隔切除术后放置宫内节育器及应用激素补充治疗对术后妊娠结局的影响[J].实用妇产科杂志,2013,29(10):769-772.

[5] 王丽平,汪沙,张颖,等.雌二醇对子宫腺肌病患者子宫内膜-肌层交界区平滑肌细胞游离Ca2+调节模式的初步研究[J].中华妇产科杂志,2012,47(5):351-354.

[6] 宁程程,单伟伟,杨冰义,等.肿瘤相关巨噬细胞(TAMs)促进Ⅰ型子宫内膜癌细胞增殖和侵袭[J].复旦学报(医学版), 2014,41(4): 447-453.

[7] 黄海玲,谭慧欢,赵琼芝,等.葛根素与雌二醇对去卵巢大鼠子宫的影响[J].重庆医学,2013,42(1):37-39.

[8] 潘欣,李广波,李晗,等.鼠伤寒沙门菌对巨噬细胞内过氧化物酶体与诱导型一氧化氮合酶结合的诱导作用[J].解放军医学杂志, 2011,36(10):1023-1026.

[9] 杨洋,徐晓玉.巨噬细胞在子宫内膜异位症中的作用研究进展[J].重庆医学,2011,40(15):1532-1534.

[10] 唐雪莲,谢梅青,赵晓苗,等.雌二醇及雌酮对人子宫内膜细胞 GJIC及连接蛋白的影响[J].中山大学学报:医学科学版, 2013, 34(3):345-350.

[11] 邵丹琪,卢丽娜,余莉萍.巨噬细胞抑制因子-1和基质金属蛋白酶- 7在子宫内膜癌组织中的表达及意义[J].广东医学, 2014,35(17): 2743-2746.

[12] Snijders AM, Langley S, Mao JH, et al. An interferon signature identified by RNA-sequencing of mammary tissues varies across the estrous cycle and is predictive of metastasis-free survival. Oncotarget. 2014;5(12):4011.

[13] Quillay H, El Costa H, Marlin R, et al. Distinct characteristics of endometrial and decidual macrophages and regulation of their permissivity to HIV-1 Infection by SAMHD1. J Virol. 2015; 89(2):1329-1339.

[14] 丁培杰,段长恩,张世杰,等.重组人生长激素对人巨噬细胞分泌 IL-1, IL-6和TNF-α的影响[J].郑州大学学报:医学版, 2011,46(5): 684-686.

[15] 杨梅,周海珍,闫敏,等.孕酮对小鼠子宫巨噬细胞数量及膜受体 CD14, CD204 表达的影响[J].解剖学报,2010(6):842-846.

[16] 丁玉达,杨箐岩,刘戈力,等.卵巢及子宫形态学检查和GnRH激发试验的关系[J].天津医药,2012,40(5):427-429.

[17] 刘勤兴,李炳奇,邵会娟,等.促孕灌注液中黄酮对小白鼠卵巢,子宫及激素的影响[J].西北农业学报,2010,(5):32-34.

[18] 镇澜,刘义,吕立群,等.17β-雌二醇对子宫内膜异位症患者在位子宫内膜间质细胞ERK1/2信号转导通路活化的影响[J].华中科技大学学报:医学版,2010,39(4):446-451.

[19] 卢燕琼,蒋斯,张洁清,等.雌二醇诱导子宫内膜癌细胞产生的VEGF和bFGF对 MAPK 通路的影响[J].中华妇产科杂志, 2014, 49(12):925.

[20] 海娜,刘义,吕立群,等.基因转染 DKK1对17β-雌二醇促进子宫内膜异位症患者在位子宫内膜间质细胞VEGF和MMP-9表达的影响[J].华中科技大学学报:医学版,2011,39(6):738-743.

[21] 李长兴,李红芳,黄金炳,等.白藜芦醇对雌二醇诱发的去卵巢大鼠子宫内膜渗出和血清VEGF的影响[J].中国现代医学杂志, 2013, (3):23-26.

[22] Lee DS, Yanagimoto UY. Expression pattems of the implantation-associated genes in the uterus during the estrous cycle in mice. J Reprod Dev. 2005;51(6):787-798.

[23] Matsumoto H, Daikoku T, Wang H. Differential expression of ezrin/radixin/moesin (ERM) and ERM-associated adhesion molecules in the blastocyst and uterus suggests their functions during implantation. Biol Reprod. 2004;70(3): 729-736.

[24] Melo M, Meseguer M, Garrido N, et al. The significance of premature luteinization in an oocyte-donmion programme. Hum Reprod. 2006;21:1503-1507.

[25] Gnainsky Y, Granot I. Local injury of the endometrium induces an inflammatory response that promotes successful implantation. J Fertil Steril. 2010;94(6):2030-2036.

[26] Papanikolaou EG, Bourgain C, Kolibianakis E, et al. Steroid receptor expression in late follicular phase endometfium in GnRH antagonist IVF cycles is already altered, indicating initiation of early luteal phase transformation in the absence of secretory changes. Hum Reprod. 2005;20(6):1541-1547.

[27] Abrahams VM, Kim YM, Straszewski SL, et al. Macrophages and apoptotic cell clearance during pregnancy. Am J Reprod Immunol. 2004;51:275-282.

[28] Curtis HS, Goulding EH, Eddy EM, et al. Studies using the estrogen receptor alpha knockout uterus demonstrate that implantation but not decidualization-associated signaling is estrogen dependent. Biol Reprod. 2002;67(4):1268-1277.

[29] 姚佳,刘淑君,彭大才,等.孕酮对雌性小白鼠不同器官中不同分子量蛋白质合成作用的影响[J].安徽农业科学,2013(6): 2490-2493.

[30] 王乾兴,郎楠,贺斌,等.孕酮回植时间对小鼠月经样模型中子宫内膜基质细胞凋亡的影响[J].中国计划生育学杂志, 2013,21(7): 441-444.