Establishment of rabbit models of chronic femoral osteomyelitis

doi:10.3969/j.issn.2095-4344.2015.49.003

Abstract

Abstract:

BACKGROUND: Establishment of an effective and stable animal model of chronic osteomyelitis is the basis of the experimental studies on treatment of chronic osteomyelitis. Previous chronic femoral osteomyelitis models are always instable and therefore not successful.
OBJECTIVE: To investigate the experimental method of establishing rabbit models of chronic femoral osteomyelitis with Staphylococcus aureus .
METHODS: Forty healthy adult New Zealand rabbits were anesthetized. After stripping periosteum located at 2 cm below the greater trochanter, two bone holes till the medullary cavity, which were connected longitudinally and overlapped partially, were drilled with a drill of 3.5 mm bore bit. Holes were sealed with sterile bone wax. Twenty rabbits from the experimental group were intramedullarily injected first with 5% sodium morrhuate (0.1mL), and then with 0.5 mL Staphylococcus aureus (1×106 cfu). Twenty rabbits from the control group were intramedullarily injected first with 5% sodium morrhuate (0.1 mL) and then with 0.5 mL normal saline. After 4 weeks, infected regions were observed, rabbit body weight and body temperature were measured and X-ray radiographs of the damaged regions were taken, bacterial culture was performed and femoral osteomyelitis lesion degree was
evaluated using the modified osteomyelitis scoring system.
RESULTS AND CONCLUSION: After 1 week of bacterial inoculation, 20 rabbits from the experimental group began to present the symptoms of increased body temperature, loss of appetite, lameness, vaccination site reaction. After 2 weeks, exudate or pus discharge in the local area were present in 13 rabbits. After 4 weeks, 19 rabbits all presented the symptoms of local swelling, pus discharge and fever. At this time period, rabbit body temperature was noticeabley increased, body weight was remarkably decreased, and modified Norden osteomyelitis scores were obviously higher in the experimental group compared with the control group. The secretion cultures were positive for Staphylococcus aureus in 14 rabbits. The success rate of modeling was 95%. These results suggest that stable and reliable rabbit models of chronic femoral osteomyelitis can be prepared by injection with Staphylococcus aureus and sodium morrhuate into marrow cavity.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

CLC Number:

R318

Chen Jin-shui, Liao Zhao-shan, Lin Song-qing, Lu Qiao-ping, Wang Ben-hai, Huang Wen-qi, Lin Yong-yang, Zhang Hui-hao. Establishment of rabbit models of chronic femoral osteomyelitis[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(49): 7885-7889.

Figures/Tables 4

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