Growth and secretion features of microencapsulated human adrenal pheochromocytoma cells in artificial cerebrospinal fluid

doi:10.3969/j.issn.2095-4344.2015.38.009

Abstract

Abstract:

BACKGROUND: Microencapsulated cells are commonly used as a tool to overcome immune rejection after subarachnoid transplantation. However, the effect of microencapsulation on the secretion of human pheochromocytoma cells is unclear.
OBJECTIVE: To observe the growth and secretion of primarily microencapsulated cultured human pheochromocytoma cells in artificial cerebrospinal fluid.
METHODS: The human pheochromocytoma tissues were digested successively to isolate human pheochromocytoma cells that were then cultured in artificial cerebrospinal fluid. Primary cells were covered with alginate-polylysine-alginate microcapsules, and then the cell morphology was observed with inverted phase contrast microscope. Levels of met-enkephalin and norepinephrine in cell culture medium were detected by enzyme-labeled immunosorbent assay (ELISA). We used cell counting kit-8 colorimetric assay to obtain the growth curve of human pheochromocytoma cells in artificial cerebrospinal fluid.
RESULTS AND CONCLUSION: Microcapsulated human pheochromocytoma cells were in suspension and the process outgrowth increased slowly. Compared with non-microcapsulated cells, the proliferation rate of microcapsulated cells increased significantly. ELISA results revealed a significant increase in the levels of met-enkephalin and norepinephrine secreted from the microencapsulated cells compared to the
non-microcapsule group. There was a wide variation in contents of met-enkephalin and norepinephrine from different tumors. These findings indicate that microencapsulated human pheochromocytoma cells can survive well and have good secretion function in artificial cerebrospinal fluid, and human pheochromocytoma cells from different tumor tissues have stable secretory function.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: PC12 Cells, Enkephalin, Methionine, Norepinephrine

CLC Number:

R318

Li Xiao, Zhang Xin-sheng, Luo Hai-jie, Luan Shuo, Wan Qing, Wu Shao-ling, Ma Chao . Growth and secretion features of microencapsulated human adrenal pheochromocytoma cells in artificial cerebrospinal fluid [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(38): 6114-6120.

Figures/Tables 3

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