Synthetic biodegradable nerve conduits for repair of injured peripheral nerve: good biocompatibility

doi:10.3969/j.issn.2095-4344.2015.25.023

Abstract

Abstract:

BACKGROUND: In the treatment of peripheral nerve injury, we can use autologous nerve or the nerve conduit of different materials.
OBJECTIVE: To explore the effects of biodegradable nerve conduit in repairing peripheral nerve injury.
METHODS: A total of 48 New Zealand white rabbits were randomly divided into autologous nerve graft group, silicone catheter group and biodegradable nerve conduit group. 10-mm sciatic nerve was obtained from each group to construct animal models of sciatic nerve defect, which was repaired with autologous nerve, silicone catheter and biodegradable nerve conduit.
RESULTS AND CONCLUSION: At 3 weeks after transplantation, motor nerve conduction and recovery rate of triceps muscle wet weight were poorer in the silicone catheter group than in the autologous nerve graft group. Motor nerve conduction and recovery rate of triceps muscle wet weight were similar between the biodegradable nerve conduit group and autologous nerve graft group. At 12 weeks, a large number of uniform myelinated nerve fiber was visible in the autologous nerve graft group. A large number of uneven myelinated nerve fiber was found in the biodegradable nerve conduit group. A small number of uneven myelinated nerve fiber was seen in the silicone catheter group. These data suggest that the synthetic biodegradable materials of nerve conduit can obtain good effect, which is close to the autologous nerve graft.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Sciatic Nerve, Nerve Fibers, Myelinated, Silica Gel, Polymers

CLC Number:

R318

Zhang Sun-fu, Wang Bin. Synthetic biodegradable nerve conduits for repair of injured peripheral nerve: good biocompatibility [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(25): 4054-4058.

Figures/Tables 4

References

[1] 张万里,王伟.甲壳素类神经导管修复周围神经的研究与进展[J].中国组织工程研究与临床康复,2009,13(47):9321-9324.

[2] 李志跃,李际才,赵群.聚乳酸聚羟基乙酸共聚物三维神经导管修复周围神经缺损[J].中国组织工程研究与临床康复,2010,14(34): 6313-6318.

[3] 向杰,黄继锋,李德忠,等.新型仿生人工神经导管诱导犬周围神经再生过程的组织学观察[J].中国组织工程研究,2012,16(21): 3801-3805.

[4] 林利,王伟莉,黄继锋,等彩色多普勒超声对PRGD复合神经导管修复周围神经缺损的评价[J].华南国防医学杂志,2014(6): 570-572.

[5] 门永芝,於子卫.生物材料构建神经导管修复周围神经损伤的研究进展[J].听力学及言语疾病杂志,2014,22(6):654-658.

[6] 王华松,吴刚,黄继锋,等.新型仿生人工神经导管修复周围神经缺损的初步临床观察[J].中国临床解剖学杂志,2014,32(6): 735-738.

[7] Jeon S, Jha MK, Ock J, et al. Role of lipocalin-2-chemokine axis in the development of neuropathic pain following peripheral nerve injury. J Biol Chem. 2013;288(33):24116- 24127.

[8] Patro N, Nagayach A, Patro IK. Iba1 expressing microglia in the dorsal root ganglia become activated following peripheral nerve injury in rats. Indian J Exp Biol. 2010;48(2):110-116.

[9] Oktay F, Cömert D, Gökkaya NK, et al. Bone age in children with obstetrical brachial plexus palsy: effect of peripheral nerve injury on skeletal maturation. J Child Neurol. 2014; 29(2):203-209.

[10] 唐圆,刘湘华,刘丹,等.以组织工程建构技术修复周围神经损伤的难点[J].中国组织工程研究,2013,17(7):1295-1304.

[11] Duan XH, Cheng LN, Zhang F, et al. In vivo MRI monitoring nerve regeneration of acute peripheral nerve traction injury following mesenchymal stem cell transplantation. Eur J Radiol. 2012;81(9):2154-2160.

[12] 李春波,陈增淦,陈统一,等.周围神经缺损修复研究进展[J].国际骨科学杂志,2013,34(6):420-423.

[13] 谢峰,李青峰,顾彬,等.神经导管修复周围神经损伤的临床应用[J].整形再造外科杂志,2004,1(3):144-146.

[14] 胡辉,张伟才,黄继锋,等.新型神经导管复合材料与骨髓间充质干细胞的相容性[J].中国组织工程研究,2013,17(16):2913-2920.

[15] 何宇,杨述华.周围神经组织工程进展[J].中华神经外科疾病研究杂志,2007,6(2):191-192.

[16] 向杰.可吸收诱导神经修复材料用于周围神经缺损的实验研究及初步临床应用[D].广州:南方医科大学,2012.

[17] Shea JE, Garlick JW, Salama ME, et al. Side-to-side nerve bridges reduce muscle atrophy after peripheral nerve injury in a rodent model. J Surg Res. 2014;187(1):350-358.

[18] 单臣.应用神经导管治疗周围神经损伤的研究进展[J].吉林医学,2009,30(23):2930-2932.

[19] 郑磊.壳聚糖神经导管及其复合骨髓间充质干细胞修复大鼠周围神经损伤的研究[D].济南:山东大学,2009.

[20] 罗鹏,彭邱亮,向剑平,等.合成材料神经导管与自体神经移植修复周围神经缺损的比较[J].中国组织工程研究,2013,17(16): 3010-3017.

[21] Nagata K, Itaka K, Baba M, et al. Muscle-targeted hydrodynamic gene introduction of insulin-like growth factor-1 using polyplex nanomicelle to treat peripheral nerve injury. J Control Release. 2014;183:27-34.

[22] 谢峰,李青峰,赵林森.几丁糖-聚乳酸复合生物材料神经导管的预构[J].组织工程与重建外科杂志,2005,1(1):42-46.

[23] 李玢,郝立君.不同神经导管材料的研究进展[J].中国美容整形外科杂志,2014,25(1):62-64.

[24] 李晓强,莫秀梅,范存义,等.神经导管研究与进展[J].中国生物工程杂志,2007,27(7):112-116.

[25] Lim H, Kim D, Lee SJ. Toll-like receptor 2 mediates peripheral nerve injury-induced NADPH oxidase 2 expression in spinal cord microglia. J Biol Chem. 2013;288(11):7572-7579.

[26] 孙晓红,佟晓杰,黄威.周围神经损伤与组织工程修复的研究进展[J].解剖科学进展,2004,10(2):140-143.

[27] 罗丽花,周毅.周围神经导管研究与进展[J].现代医药卫生,2015, 31(4):523-525.

[28] 杨华清,张志军,王建华,等.神经导管结合神经片段修复外周神经损伤[J].中华临床医师杂志(电子版),2011,5(19):5594-5598.

[29] Cirillo G, Colangelo AM, Bianco MR, et al. BB14, a Nerve Growth Factor (NGF)-like peptide shown to be effective in reducing reactive astrogliosis and restoring synaptic homeostasis in a rat model of peripheral nerve injury. Biotechnol Adv. 2012;30(1):223-232.

[30] 胡辉.新型神经导管复合材料与骨髓间充质干细胞用于周围神经缺损的基础研究[D].广州:南方医科大学,2013.

[31] 殷湘慧.用于修复周围神经损伤的复合材料及其性能研究[D].武汉:武汉理工大学,2005.

[32] 向杰.人工神经导管基础与临床研究进展[J].中国临床解剖学杂志,2012,30(5):587-589.

[33] 陈家玉,刘文和,曹锡文,等.周围神经损伤与修复[J].湘南学院学报(医学版),2009,11(3):1-4.

[34] Morisaki S, Ota C, Matsuda K, et al. Application of Raman spectroscopy for visualizing biochemical changes during peripheral nerve injury in vitro and in vivo. J Biomed Opt. 2013; 18(11):116011.

[35] Rochkind S, Drory V, Alon M, et al. Laser phototherapy (780 nm), a new modality in treatment of long-term incomplete peripheral nerve injury: a randomized double-blind placebo-controlled study. Photomed Laser Surg. 2007;25(5): 436-442.

[36] 陈令军,喻锦成,符俏,等.生物材料构建神经导管修复周围神经损伤的临床应用[J].中国组织工程研究,2012,16(34):6413-6420.

[37] Arslantunali D, Budak G, Hasirci V. Multiwalled CNT-pHEMA composite conduit for peripheral nerve repair. J Biomed Mater Res A. 2014;102(3):828-841.

[38] Benzina O, Szabo V, Lucas O, et al. Changes induced by peripheral nerve injury in the morphology and nanomechanics of sensory neurons. J Biomed Opt. 2013;18(10):106014.

[39] Huang J, Zhang Y, Lu L, et al. Electrical stimulation accelerates nerve regeneration and functional recovery in delayed peripheral nerve injury in rats. Eur J Neurosci. 2013; 38(12):3691-3701.

[40] 杨华林.神经导管生物材料修复周围神经损伤的动物实验[J].中国组织工程研究与临床康复,2010,14(29):5439-5442.

[41] 张伟,岳靓,易红蕾,等.自组装多肽凝胶联合可降解神经导管修复长节段周围神经损伤[J].中国矫形外科杂志,2011,19(24): 2083-2087.

[42] 刘勇,侯春林.神经导管研究进展[J].国际骨科学杂志,2010,31(5): 279-281.

[43] 赵文.新型复合材料神经导管促进周围神经再生的研究[D].上海:上海交通大学,2007.

[44] 张伟,侯春林.神经移植术修复周围神经损伤[J].国外医学(骨科学分册),2002,23(2):98-101.

[45] 魏国宝,朱康杰.神经导管修复周围神经损伤的研究进展[J].生物医学工程学杂志,2001,18(4):653-657.

[46] 张飞.应用神经导管治疗周围神经损伤的研究进展[J].医学综述, 2014,20(11):1997-1999.

[47] 谢峰,李青峰,赵林森,等.新型复合生物材料导管修复大鼠周围神经缺损的实验研究[J].中华整形外科杂志,2005,21(4):295-298.

[48] 徐海栋,赵建宁.组织工程化神经导管修复外周神经损伤[J].中国组织工程研究,2013,17(12);2205-2209.

[49] 庄友梅.神经导管材料应用于神经损伤修复的研究进展[J].广东牙病防治,2012,20(11):605-608.

[50] 李政,周明.神经导管联合自体变性肌桥修复大鼠周围神经缺损的实验研究[J].组织工程与重建外科,2012,8(3):146-149.

[51] 刁云锋,商崇智.神经导管生物材料在神经修复中的应用[J].中国组织工程研究与临床康复,2011,15(34):6424-6427.

[52] 胡文.组织工程化神经修复神经缺损的临床研究进展[J].交通医学,2010,24(6):596-598.

[53] 张凤久,赵志英,刘启,等.神经导管修复大鼠坐骨神经缺损实验研究[J].包头医学院学报,2010,26(3):14-16.

[54] 赵红斌,马敬,杨银书,等.胶原神经导管对外周神经缺损修复的实验研究[J].生物医学工程与临床,2010,14(2):100-104.