Ganglioside effect on Nogo-A expression of rat oligodendrocytes in vitro after carbon monoxide poisoning

doi:10.3969/j.issn.2095-4344.2015.06.022

Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (6): 945-949.doi: 10.3969/j.issn.2095-4344.2015.06.022

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Ganglioside effect on Nogo-A expression of rat oligodendrocytes in vitro after carbon monoxide poisoning

Wang Xiao-hong1, Wang Hong2, Che Ju-hua4, Wang Su-ping1, Wang Tao3, Zhu Yan-ling5

1Neurology Department, 2Comprehensive Ward, 3Rehabilitation Ward, Dalian Municipal Central Hospital, Dalian 116033, Liaoning Province, China; 4Dalian Medical University, Dalian 116044, Liaoning Province, China; 5Department of Neurology, the Third People’s Hospital of Dalian, Dalian 116033, Liaoning Province, China

Received:2015-01-13 Online:2015-02-05 Published:2015-02-05
Contact: Wang Hong, Master, Chief physician, Comprehensive Ward, Dalian Municipal Central Hospital, Dalian 116033, Liaoning Province, China
About author:Wang Xiao-hong, Master, Associate chief physician, Neurology Department, Dalian Municipal Central Hospital, Dalian 116033, Liaoning Province, China
Supported by:
the Scientific Research Plan of Dalian Health Department in 2008

Abstract

Abstract:

BACKGROUND: Nogo-A protein is speculated to have close relationship to delayed encephalopathy after carbon monoxide poisoning because it is a kind of neurite growth inhibitor expressing in oligodendrocytes. Monosialotetrahexosylganglioside can improve nerve damage after carbon monoxide poisoning, but no reports is concerned about whether the neuroprotective effect of ganglioside is related to Nogo-A protein.
OBJECTIVE: To investigate the protective mechanism of ganglioside on nerve cells after carbon monoxide poisoning from the perspective of Nogo-A in oligodendrocytes.
METHODS: Rat oligodendrocytes cultured in vitro were divided into three groups: control, carbon monoxide and monosialotetrahexosylganglioside groups. Carbon monoxide and monosialotetrahexosylganglioside groups were cultured in an airtight box containing 1% carbon monoxide. 5 mg/L monosialotetrahexosylganglioside was added into the culture medium of monosialotetrahexosylganglioside group in advance. The expression of Nogo-A mRNA and protein at 6, 24, 48 hours were detected by RT-PCR and immunohistochemistry method, respectively.
RESULTS AND CONCLUSION: The integrated absorbance values of Nogo-A mRNA and cumulative
absorbance values of Nogo-A protein in the carbon monoxide group at 6, 24 and 48 hours were significantly higher than those in the control group (P < 0.05). At 24 hours after carbon monoxide treatment, the integrated absorbance value and cumulative absorbance value of Nogo-A were both higher than those in the control group (P < 0.05), but lower than those in the carbon monoxide group (P < 0.05). The integrated absorbance values of Nogo-A mRNA was significantly associated with the cumulative absorbance values of Nogo-A protein (r=0.95). These findings indicate that carbon monoxide can activate oligodendrocytes, and raise the transcription and translation levels of Nogo-A. The increase expression of Nogo-A protein was due to the high level of Nogo-A mRNA. The protective mechanism of ganglioside on nerve cells after carbon monoxide poisoning is related to inhibition of Nogo-A expression in oligodendrocytes.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Carbon Monoxide Poisoning, Brain Diseases, Oligodendroglia

CLC Number:

R394.2

Wang Xiao-hong, Wang Hong, Che Ju-hua, Wang Su-ping, Wang Tao, Zhu Yan-ling . Ganglioside effect on Nogo-A expression of rat oligodendrocytes in vitro after carbon monoxide poisoning[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(6): 945-949.

Figures/Tables 2

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Ganglioside effect on Nogo-A expression of rat oligodendrocytes in vitro after carbon monoxide poisoning

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