In vitro biocompatibility and bioactivity of a new medical Mg-Li-Ca alloy

doi:10.3969/j.issn.2095-4344.2014.52.009

Abstract

Abstract:

BACKGROUND: Whether the new type of Mg-Li-Ca alloy has good biocompatibility and bioactivity or not has not been confirmed.

OBJECTIVE: To evaluate the in vitro biocompatibility and bioactivity of a new medical Mg-Li-Ca alloy, and to find the possibility of this Mg-Li-Ca alloy as a new type of implant material.

METHODS: The MC3T3-e1 (mouse osteoblasts) was used to be the test cells, then cultured and subcultured in vitro at passage 3. The cells were cultured and developed by leaching liquors of Mg-Li-Ca alloy, pure magnesium, and AZ31B alloy, and α-minimum essential medium, respectively. MTT assay was used to detect the absorbance values at days 1, 3, 5 of culture, and then the relative growth rate was calculated. Alkaline Phosphatase Colorimetric Assay Kit was used to check the activity of alkaline phosphatase at days 5 and 7 of culture. And the MC3T3-e1 cells were cocultured with Mg-Li-Ca alloy, pure magnesium, and AZ31B alloy, respectively; and at 1 day of coculture, cell adhesion and proliferation was observed under scanning electron microscopy.

RESULTS AND CONCLUSION: Mg-Li-Ca alloy, pure magnesium, and AZ31B alloy were non-cytotoxic to osteoblasts, and the cytotoxicity of Mg-Li-Ca alloy was lower than that of pure magnesium and AZ31B alloy, indicating Mg-Li-Ca alloy had no influence on osteoblast proliferation and had good biocompatibility. The alkaline phoaphatase activity was not influenced by Mg-Li-Ca alloy and pure magnesium that had good bioactivity. AZ31B had a significant impact on the normal synthesis of alkaline phosphatase in osteoblasts. The MC3T3-e1 cells can adhere and grow normally on the surface of Mg-Li-Ca alloy, pure magnesium, and AZ31B alloy. These findings indicate that the new type of mg-Li-Ca alloy is expected to be a new bone implant material.

Key words: materials testing, alloys, osteoblasts

CLC Number:

R318

Liu Rui, Jiang Ke, Zhao Bao-dong, Zeng Rong-chang, Xu Hao, Wang Lan-ying. In vitro biocompatibility and bioactivity of a new medical Mg-Li-Ca alloy[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(52): 8409-8413.

Figures/Tables 2

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