Unilateral olfactory functional deprivation model in the left peripheral nostrils by electric cautery injury: olfactory bulb neurogenesis and transformation

doi:10.3969/j.issn.2095-4344.2014.02.012

Abstract

Abstract:

BACKGROUND: Olfactory bulb neurogenesis, transformation and maturation have been considered the hot topic. Olfactory bulb experience and nervous activity can influence olfactory bulb neurogenesis. However, no study reports that olfactory bulb functions can affect olfactory bulb neurogenesis in guinea pigs.
OBJECTIVE: To investigate the effect of unilateral olfactory functional deprivation on doublecortin, calbindin and parvalbumin expression in olfactory bulb of juvenile guinea pigs.
METHODS: Totally 24 guinea pigs were randomly divided into two groups, which were killed after establishing olfactory functional deprivation model through electric cautery injury at the left peripheral nostrils. At 3 and 6 weeks after modeling, the specimens were harvested. The expression change of doublecortin, calbindin and parvalbumin in two sides’ olfactory bulb of juvenile guinea pigs was detected by immunohistochemistry.
RESULTS AND CONCLUSION: The number of doublecortin, calbindin and parvalbumin positive cells in olfactory bulb at the un-deprived side was significantly higher than that at the deprived side at 3 and 6 weeks (P < 0.05). This finding indicates that olfactory neural activities can affect neurogenesis and transformation in guinea pigs.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: olfactory sensation, olfactory bulb, microtubular proteins, calbindin, vitamin D-dependent, parvalbumin

CLC Number:

R318

Zeng Jian-jun, He Xu, Yan Xiao-xin, Luo Xue-gang, Pan Ai-hua. Unilateral olfactory functional deprivation model in the left peripheral nostrils by electric cautery injury: olfactory bulb neurogenesis and transformation[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(2): 231-238.

Figures/Tables 4

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