Biocompatibility of nano-TiO2:C thin film coating

doi:10.3969/j.issn.2095-4344.2013.08.013

Abstract

Abstract:

BACKGROUND: In previous experiments, TiO₂:C thin film coating have been prepared on the surface of titanium paper by sol-gel process.
OBJECTIVE: To evaluate the biocompatibility of nanometer TiO₂:C thin film coating.
METHODS: Hemolysis test: New Zealand white rabbit venous blood was extracted and added into the TiO₂-normal saline, TiO₂:C-normal saline, distilled water and fresh normal saline. Cytotoxicity test: TiO₂:C leaching liquor was added into the L929 cell culture medium in the logarithmic growth phase. Short-term systemic toxicity test: 60 BALB/C mice were randomly divided into three groups and lavaged with leaching liquor extracted from TiO₂, TiO₂:C coatings and normal saline. Oral mucosa stimulation test: the gutta-percha wafer and the nano-TiO2:C thin film coating were sutured and fixed to the cheek pouch of inaurate hamster.
RESULTS AND CONCLUSION: Acute hemolysis was not induced by nano-TiO₂:C thin film coating. Toxicity level of nano-TiO₂:C thin film coating was 0-1. It had no obvious toxicity to the cells. There was no obvious abnormality of cell cycle. TiO₂:C leaching liquor had no short-term systemic toxicity on mice and no stimulation to oral mucosa of hamster. The results showed that nano-TiO₂:C thin film coatings have good bio-security.

Key words: biomaterials, nanobiomaterials, material biocompatibility, nano-TiD2, C, cytotoxicity, biocompatibility, coating, the National Natural Science Foundation of China, biomaterial photographs-containing paper

CLC Number:

R318

Mai Li-xiang, Zhang Sheng, Xie Yong-jian, Xie Bin, Wang Da-wei, Zhang Zhi-guang. Biocompatibility of nano-TiO2:C thin film coating[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(8): 1404-1410.

Figures/Tables 9

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