Effect of Uygur Medicine Maizhuni on inflammatory factors in the articular cartilage following osteoarthritis

doi:10.3969/j.issn.2095-4344.2013.07.005

Abstract

Abstract:

BACKGROUND: Interleukin-1β is considered as an important cytokine for improvement of cartilage matrix degradation and destruction of articular cartilage during the pathological process of osteoarthritis.
OBJECTIVE: To establish the expression of interleukin-1β in the articular cartilage of osteoarthritis rats and to study the effect of Uygur Medicine Maizhuni.
METHODS: Forty healthy Sprague Dawley rats were randomly assigned into four groups: Maizhuni intervention group, model control group, shame operation group, and normal control group. Knee osteoarthritis models were established in the model control and Maizhuni intervention groups using modified Hulth method. In the sham operation group, only the knee was exposed with no ligament resection and medial meniscectomy. Rats in the Maizhuni intervention group were treated with Uygur Medicine Maizhuni (10.31 mg/(kg•d)) and those in the model control and sham operation groups were administrated with saline by gavage at week 2 after model establishment. The treatment was continued for 4 weeks.
RESULTS AND CONCLUSION: The degree of cartilage degeneration in the model control group was higher than that in the Maizhuni intervention group as observed by naked eye. General score and Mankin score in the model group were significantly higher than those in the Maizhuni intervention group (P < 0.05). There was significantly greater expression of interleukin-1β in the chondrocytes of the model control group (P < 0.05). Sham operation group did not differ from the normal control group in general score, Mankin score and interleukin-1β expression in the chondrocytes (P > 0.05). These findings indicate that Uyghur Medicine Maizhuni may protect the articular cartilage by inhibiting the expression of interleukin-1β.

Key words: tissue construction, cartilage tissue construction, osteoarthritis, arthritis, knee, articular cartilage, proinflammatory factor, interleukin-1 beta, interleukin-1, Uygur medicine Maizhuni, Mankin score, chondrocytes, provincial grants-supported paper, tissue construction photographs-containing paper

CLC Number:

R318

Hong Han-gang, Zhang Yao-wu, Niu Qing-hai, Fang Rui, Meng Qing-cai. Effect of Uygur Medicine Maizhuni on inflammatory factors in the articular cartilage following osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(7): 1162-1167.

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