Variation of RhoA/ROCK transduction pathway in the articular cartilages under abnormal stress

doi:10.3969/j.issn.2095-4344.2013.02.009

Abstract

Abstract:

BACKGROUND: In order to confirm whether RhoA/ROCK transduction pathway play an active role in in vivo articular cartilage degeneration or osteoarthritis, we designed this experiment.
OBJECTIVE: To explore the role of the RhoA/ROCK transduction pathway in the process of articular cartilage degeneration.
METHODS: Totally 36 New Zealand white rabbits were randomly divided into a control and 2-, 4- and 6-week immobilization groups, and the right hindlimbs of rabbits were immobilized with plaster bandage in extension position except those in the control group. The articular cartilage of the lateral tibial plateau was harvested for hematoxylin-eosin staining and immunohistochemistry to observe the pathological changes and the content of RhoA and ROCK.
RESULTS AND CONCLUSION: The articular cartilage degenerated with time prolonged, and the Mankin scores showed there were significant differences among groups (P < 0.05). Immunohistochemical results showed that the RhoA/ROCK transduction pathway was motivated mainly in the tangent layer and the middle layer of the cartilage and inhibited with immobilization time prolonged. The RhoA/ROCK transduction pathway takes an important role in the process of articular cartilage degeneration.

Key words: tissue construction, cartilage tissue construction, abnormal stress, articular cartilage, RhoA, ROCK, immunohistochemistry, immobilization, tissue construction photographs-containing paper

CLC Number:

R318

Zhang Hai-xiang, Wang Chen. Variation of RhoA/ROCK transduction pathway in the articular cartilages under abnormal stress[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(2): 235-240.

Figures/Tables 5

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