Condensed autologous bone marrow transplantation facilitates the tendon-bone healing

doi:10.3969/j.issn.2095-4344.2013.02.007

Abstract

Abstract:

BACKGROUND: There are a lot of applications of hamstring tendons autografts in the reconstruction of anterior cruciate ligament, which needs a long term for the tendon-bone healing and restricts the early functional activities of patients.
OBJECTIVE: To evaluate the effect of condensed autologous bone marrow transplantation on the tendon-bone interface in the reconstruction of anterior cruciate ligament.
METHODS: One knee joint was chosen as the experimental group and the other side as the control group in 32 New Zealand rabbits experiencing the reconstruction of anterior cruciate liagment. The tibial tendon-bone interface was injected with condensed autologous bone marrow in the experimental side and the other side was not injected as the control.
RESULTS AND CONCLUSION: The histological anatomy showed the tendon-bone interface was filled with granulation tissues that were loose, and there were some activities of bone formation in the control group at 2 weeks after operation; the tendon-bone interface was filled with granulation tissues that were not compact, but there were active activities of bone formation in the experimental group at 2 weeks after operations. The bound between the tendon and bone was becoming obscure, and there were collagen fiber-fibrocartilage-calcified fibrocartilage-bone transitional zones at the interface in the control group at 12 weeks after operation. In the experimental group, there were conspicuous collagen fiber-fibrocartilage-calcified fibrocartilage-bone transitional zones and clear tidal line at the interface. The maximal pull-out loads in the experimental group were higher than those in the control group at 4, 8 and 12 weeks after operation (P < 0.05). These findings indicate that condensed autologous bone marrow transplantation can enhance the tensile strength of tendon-bone interface, and can do good to tendon-bone healing in a bone tunnel.

Key words: tissue construction, bone tissue construction, autologous bone marrow, transplantation, cruciate ligament, knee, tendon-bone healing, tissue engineering, tissue construction photographs-containing paper

CLC Number:

R318

Zhang Yi-long, Li Ning, Song You-xin, Fang Ya-qun, Zhang Hong, Zhang Jin-yan. Condensed autologous bone marrow transplantation facilitates the tendon-bone healing[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(2): 223-227.

Figures/Tables 5

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