Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (20): 3768-3772.doi: 10.3969/j.issn.1673-8225.2012.20.036

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Human apM1 gene transfers into the epicardial adipose tissue to inhibit the formation of coronary atherosclerosis in rabbits

Yang Wen-kai1, 2, Dong Nian-guo1, Chen Qing3, Wang Xian-guo1, Xie Ting1, Chen Xin-zhong1   

  1. 1Department of Cardiac Surgery, 3Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan  430022, Hubei Province, China; 2Department of Cardiac Surgery, Jiangxi Provincial People’s Hospital, Nanchang  330006, Jiangxi Province, China
  • Received:2012-01-19 Revised:2012-02-28 Online:2012-05-13 Published:2012-05-13
  • Contact: Chen Xin-zhong, Doctor, Attending physician, Department of Cardiac Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China xinzhongchen@yahoo.com.cn
  • About author:Yang Wen-kai☆, Studying for doctorate, Attending physician, Department of Cardiac Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China; Department of Cardiac Surgery, Jiangxi Provincial People’s Hospital, Nanchang 330006, Jiangxi Province, China doctor_wenkaiyang@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30872541*; Doctoral Foundation for New Teachers, No. 200804871116*

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Abstract:

BACKGROUND: The decreased level of adiponectin secreted from the epicardial adipose tissue may be one of the main reasons leading to coronary atherosclerosis.
OBJECTIVE: To investigate the effect of human apM1 gene transferring into the epicardial adipose tissue on the formation of coronary atherosclerosis in high-cholesterol fed rabbits.
METHODS: 50 μL mixture of pEGFP-apM1 and liposome was injected into the rabbit pericardial cavity. The epicardial adipose tissue was harvested at 2, 7 and 28 days respectively after injection and was made into fast frozen sections. The transfection efficiency and expression of apM1 were detected by fluorescence microscopy and immunohistochemical method. Twenty-four male New Zealand White rabbits were randomly divided into four groups: normal control group, high-fat group, high-fat plus pEGFP injection group, high-fat plus pEGFP-apM1 injection group. At 4 weeks after injection, the expression of apM1 in epicardial adipose tissue was detected by Western blot analysis.
RESULTS AND CONCLUSION: In the rabbit epicardial adipose tissue, the high expression of apM1 was detected at 2 days after injection and until to 28 days. The effective transfection was not detected in the epicardial connective and myocardial tissues. The levels of adiponectin, tumor necrosis factor-α, total cholesterol, high density lipoprotein cholesterol and low density lipoprotein cholesterol in the peripheral blood changed insignificantly change after pEGFP-apM1 injection into the pericardial cavity. In high-fat plus pEGFP-apM1 injection group, the distinct increase of adiponectin and decrease of tumor necrosis factor-α (P < 0.01) were measured in the pericardial cavity fluid. Compared with the high-fat group, pEGFP-apM1 injection could decrease the intima/media ratio of left coronary artery to 40.66%. The apM1 gene could be effectively transferred into the epicardial adipose tissue through the pericardial cavity injection. The increase of adiponectin expression in the epicardial adipose tissue could inhibit local inflammatory cytokines release induced by high-fat feeding and inhibit the formation of coronary atherosclerosis.

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