Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (20): 3735-3738.doi: 10.3969/j.issn.1673-8225.2012.20.028

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Protection effect of Gastrodin on chemical damage of skeletal muscle cells in rats  

Yang Yu-qin, Zhu Dao-li, Chen Pei-lin, Yu Chun-mei, Yang Yan-hua   

  1. School of Life Science, Nantong University, Nantong  226019, Jiangsu Province, China
  • Received:2011-10-15 Revised:2011-12-26 Online:2012-05-13 Published:2012-05-13
  • Contact: Zhu Dao-li, Professor, School of Life Science, Nantong University, Nantong 226019, Jiangsu Province, China zhudaoli@ntu.edu.cn
  • About author:Yang Yu-qin, School of Life Science, Nantong University, Nantong 226019, Jiangsu Province, China
  • Supported by:

    College Students Innovative Training Program Fund Project of Nantong University, No. 2010038*; Doctoral Special Construction of Nantong University, No. 05024276*

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Abstract:

BACKGROUND: Modern pharmacological studies have showed that Gastrodin can restore the imbalance between excitability and inhibition of cerebral cortex, produce sedation, sleep, analgesia and other central inhibition.
OBJECTIVE: To investigate the protective effect of Gastrodin on chemical damage of skeletal muscle cells in L6 rats.
METHODS: A chemical myoblast damage rat model of L6 was established by using H2O2. Samples of pre-protective group were cultured in Dulbecco's modified Eagle’s medium (DMEM) containing different concentrations of Gastrodin (1.562 500, 0.781 250, 0.390 625 g/L) for a 24-hour pretreatment, followed by the incubation of H2O2 (0.1 mmol/L) for 80 minutes. At the same time, the proliferation group (different concentrations of Gastrodin for a 24-hour pretreatment, but not followed by the incubation of H2O2), model group (only the incubation of H2O2), positive control group (only L6 rat myoblast suspension), negative control group (only DMEM medium) and control group (without Gastrodin pretreatment and H2O2 incubation) were established.
RESULTS AND CONCLUSION: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that the survival rate of the proliferation and pre-protective groups was significantly higher than that of the model group. Cell nuclei were labeled by DAPI turned blue, while the fluorescent intensity of DAPI in the model group was obviously much lower than that in the pre-protective group and so was the quantity of cells labeled by DAPI. Cell nuclei were more regularly shaped, more compact cytoplasm and clearer cell outline in the pre-protective group from hematoxylin-eosin staining. However, cells with structural damages were obviously observed in the model group. Bax and Bcl-2 immunofluorescent cytochemical technique demonstrated that the expression of bax in the model group was evidently higher than that in the pre-protective group, while the expression of bcl-2 was the exact opposite. According to the results of flow cytometry, the rate of apoptosis in the pre-protective group was significantly lower than that in the model group (P < 0.05). It is indicated that Gastrodin has remarkable effect on injury of skeletal muscle cells in rats induced by chemicals.

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