Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (20): 3690-3693.doi: 10.3969/j.issn.1673-8225.2012.20.018

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Establishment of an experimental atherosclerosis model by feeding high-fat diet plus injecting bovine serum albumin in rabbits

Li Lin, Dou Jian-lin, Chu Tian-shu, Li Wan-qiu, Zhang Ge, Sun Lin   

  1. Department of Cardiology, the Second Affiliated Hospital of Kunming Medical University, Kunming  650101, Yunnan Province, China
  • Received:2012-03-01 Revised:2012-04-05 Online:2012-05-13 Published:2012-05-13
  • Contact: Sun Lin, Doctor, Associate professor, Master’s supervisor, Department of Cardiology, the Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan Province, China sunlinkm@sina.com
  • About author:Li Lin★, Master, Department of Cardiology, the Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan Province, China 751065905@qq.com
  • Supported by:

     the General Foundation of Yunnan Provincial Applied Basic Research, No. 2008CD122*; the Science and Technology Research Foundation of Yunnan Provincial Education Bureau, No. 2010Z034*

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Abstract:

BACKGROUND: It is commonly seen to establish a model of atherosclerotic stenosis by simply feeding high-fat diet.
OBJECTIVE: To establish an atherosclerosis model by feeding high-fat diet plus injecting bovine serum albumin in rabbits.
METHODS: Three different methods were used to establish rabbit models of atherosclerosis, including simple high-fat diet, high-fat diet+lipid milk, and high-fat diet+bovine serum albumin. Normal control group was fed with normal food.
RESULTS AND CONCLUSION: The levels of serum total cholesterol, triglyceride, low density lipoprotein and high density lipoprotein were significantly increased in the former three groups compared with normal control group (P < 0.01). The rabbits treated with simple high-fat diet possibly presented with hyperlipidemia rather than atherosclerotic lesions. The rabbits treated with high fatty feedstuff only showed hyperlipidemia without atherosclerosis lesion. The rabbits treated with high-fat diet and lipid milk showed fibrous plaque. The mature atherosclerotic plaques were presented in the rabbits treated with high-fat diet+bovine serum albumin. The mature atherosclerotic plaque may be created by high-fat diet plus bovine serum albumin that an ideal model of atherosclerosis can be established in rabbits.

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