Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (20): 3675-3679.doi: 10.3969/j.issn.1673-8225.2012.20.015

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Effects of dragon’s blood on the expression of substance P and Bcl-2 in wound tissue of diabetic scalded rats  

Zhang Xian-fa1, Wu Zheng-qiu2, Liang Zi-qian1, Zhao Xue-kai1, Ding Hua-rong1   

  1. 1Department of Burns and Plastic Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China; 2 Department of Traditional Chinese Medicine Surgery, Binyang People’s Hospital, Nanning  530405, Guangxi Zhuang Autonomous Region, China
  • Received:2011-11-02 Revised:2012-01-10 Online:2012-05-13 Published:2012-05-13
  • Contact: Wu Zheng-qiu, Department of Traditional Chinese Medicine Surgery, Binyang People's Hospital, Nanning 530405, Guangxi Zhuang Autonomous Region, China wzhq6767@163.com
  • About author:Zhang Xian-fa★, Studying for master’s degree, Department of Burns and Plastic Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China zxiana2003@163.com
  • Supported by:

    Graduate Education Innovation Fund of Guangxi Zhuang Autonomous Region, No. 2010105981002 M187* 
       

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Abstract:

BACKGROUND: Previous studies have demonstrated that dragon’s blood can promote the wound healing as well as have characteristics of anti-platelet aggregation, anti-inflammatory analgesic, anti-bacterial and antioxidant and other biological activity.
OBJECTIVE: To identify the effects of dragon’s blood on the expression of substance P and Bcl-2 in wound tissue of diabetic scalded rats.
METHODS: A total of 112 Wistar rats were randomly divided into four groups: dragon’s blood group, silver sulfadiazine (SD-Ag) group, diabetic control group and normal control group. Diabetic deep Ⅱ degree burn models were established in dragon’s blood group, SD-Ag group and diabetic control group, and the normal deep Ⅱ degree burn models were established in normal control group.
RESULTS AND CONCLUSION: On the 7th day post scald, the wound healing rates of dragon’s blood group and normal control group were higher than those of SD-Ag group and diabetic control group (P < 0.05). After 21 days, the wound healing rate of dragon’s blood group was significantly higher than that of SD-Ag group and diabetic control group (P < 0.05) and highest in normal control group. After 15 days, the positive expression level of substance P reached a peak in normal control group and was higher than other three groups, and then decreased gradually. The expression was most positive in dragon’s blood group, SD-Ag group and diabetic control group at the 21th day post scald, and which was significantly higher in dragon’s blood group (P < 0.05). On the 3rd day after scald, the expression level of Bcl-2 in the normal control group was significantly higher than that in other groups. From the 15th day, the expression of Bcl-2 was gradually increased in each group and reached to peak, and highest in dragon’s blood group (P < 0.05), but there was no significant difference between dragon’s blood group and normal control group (P > 0.05). The results demonstrate that dragon's blood can promote the healing of diabetic burn wounds significantly by regulating the expression of substance P and Bcl-2.

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