Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (20): 3667-3670.doi: 10.3969/j.issn.1673-8225.2012.20.013

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Effect of botulinum toxin type A on the proliferation and collagen synthesis of human hypertrophic scar fibroblasts 

Li Wei-hua1, Li De-shui1, Gao Yu-wei2, Xing Yu-xi1   

  1. 1Department of Burn and Plastic Surgery, the Affiliated Hospital of Medical College of Chinese People’s Armed Police  Force, Tianjin  300162, China; 2Department of Plastic Surgery, Xingtai Boai Hospital, Xingtai  054001, Hebei Province, China
  • Received:2011-09-06 Revised:2011-11-07 Online:2012-05-13 Published:2012-05-13
  • About author:Li Wei-hua★, Master, Associate chief physician, Department of Burn and Plastic Surgery, the Affiliated Hospital of Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China lwh_689@yahoo.com.cn

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Abstract:

BACKGROUND: Local injection of botulinum toxin type A (BTA) around the wound can reduce the scar formation. The scar hyperplasia and contracture can also be inhibited; thereby the scar can become atrophic and flat.
OBJECTIVE: To investigate the effect of BTA on the proliferation and collagen synthesis at human hypertrophic scar fibroblasts.
METHODS: Human hypertrophic scar fibroblasts were isolated and cultured in vitro. The cells at logarithmic growth phase were cultured. Dulbecco's modified Eagle medium (DEME) containing BTA (diluted concentration of 0.1 U/L) was used to intervene the cell growth. Control group used DEME containing fetal bovine serum.
RESULTS AND CONCLUSION: At 1 to 15 days after cells seeding, the hypertrophic scar fibroblasts showed the spindle-like shape with uniform composition and strong proliferation, the fibroblasts proliferated into monolayers, and the hypertrophic cells showed a high degree of consistency in the control group. The cells in the BTA group grew slowly and the arrangement of cells was scattered. The amount of cells in the BTA group was 79.3% of that in the control group. The collagen synthetic rate of BTA group was 48.4% of that in the control group. It is indicated that BTA can inhibit the proliferation and collagen synthesis of human hypertrophic scar fibroblasts.

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