Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (11): 2067-2070.doi: 10.3969/j.issn.1673-8225.2012.11.039

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Application of chicken embryo in biomedical research

Wang Yong, Li Li, Li Tao, Duan Yong-gang   

  1. Key Laboratory of Cell Engineering of Luoyang, First Affiliated Hospital, Henan University of Science and Technology, Luoyang  471003, Henan Province, China
  • Received:2011-07-29 Revised:2011-11-09 Online:2012-03-11 Published:2012-03-11
  • Contact: author: Li Li, Associate chief physician, Key Laboratory of Cell Engineering of Luoyang, First Affiliated Hospital, Henan University of Science and Technology, Luoyang 471003, Henan Province, China lyjxlly@163.com
  • About author:Wang Yong★, Studying for master’s degree, Attending physician, Key Laboratory of Cell Engineering of Luoyang, First Affiliated Hospital, Henan University of Science and Technology, Luoyang 471003, Henan Province, China wangyong097@163.com
  • Supported by:

    the Natural Science Foundation of Education Bureau of Henan Province, No. 2010A310002*

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Abstract:

BACKGROUND: In recent years, there are a lot of researches on tissue culture and cell culture using chicken embryo. But neurocyte culture using chicken embryo has not been reported.
OBJECTIVE: To summarize the research progress of tissue culture and cell culture using chicken embryo in order to find a convenient and inexpensive method of neurocyte culture.
METHODS: An online search of CNKI and PubMed databases was performed for articles related to chicken embryo culture model and neurocyte culture published between January 1998 and June 2011, using key words of “chicken embryo chorioallantoic membrane model; neuronal culture” in Chinese and English. Articles related to chicken embryo culture model were selected. As to the in identical field, articles published recently or in authoritative magazine were of priority. A total of 205 articles were collected in the initial search, 34 of them were retained according to the inclusion criteria.
RESULTS AND CONCLUSlON: Chicken embryo is widely used in virus isolation, virus cultivation, vaccine preparation, mechanisms of tumor biology and drug screening. Compared with other culture methods, the chicken embryo culture model is cheap, convenient and rapid. Fully understanding and correctly utilization of the chicken embryo culture model provide scientific and feasible methods and train of thought for the research in many fields.

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