Abstract:

BACKGROUND: Under different culture and cytokine-stimulated conditions, steoblasts have different proliferation and differentiation abilities as well as functions. 
OBJECTIVE: To summarize the progress in in vitro culture of osteoblasts as well as proliferative ability of osteoblasts.
METHODS: A search of PubMed and Wanfang databases (1990/2011) was performed for articles addressing exercise, osteoblast culture and bone tissue engineering. The keywords were “exercise, osteoblast raise method” in English and “exercise, osteoblast, bone tissue engineering” in Chinese. Unrelated articles or repetitive articles were excluded, and finally 29 articles were included in result analysis.
RESULTS AND CONCLUSION: Osteoblast culture is influenced by the following factors: methods to choose seed cells, pattern of scaffold materials, cytokines, culture environment, and mechanical factors in bone tissue engineering construction. Osteoblasts cultured in different conditions and stimulated by different cytokines have different proliferative and differentiated abilities. The proliferative speed of osteoblasts is closely related to a low shear environment produced by rotating-wall vessel bioreactor, appropriate cytokines and selection of cytoskeleton.