Abstract:

BACKGROUND: Different ways to supply hydrogen have curative effect on ischemia-reperfusion injury. But the relevant metabolism studies are rare to compare the different ways of supplying hydrogen.
OBJECTIVE: To investigate the effect of intraperitoneal or intravenous saturated hydrogen saline and intraperitoneal injection of hydrogen on hydrogen metabolism in rabbits, providing a more optimal hydrogen treatment proposal.
METHODS: Twenty New Zealand white rabbits were tested the basis concentration of exhaled hydrogen. Then all of them were supplied saturated hydrogen saline or hydrogen gas 10 mL/kg via intraperitoneal injection and saturated hydrogen saline       10 mL/kg via intravenous injection within 10 minutes, respectively. Exhaled hydrogen concentrations were monitored continuously. When the exhaled concentration declined to the baseline and stabilized for more than 30 minutes, hydrogen was supplied by another way. Time to maximal exhaled hydrogen concentration and max exhaled hydrogen concentration were recorded, as well as half-life and area under the curve were calculated to compare the effects of three different ways of hydrogen supply on hydrogen metabolism in rabbits.
RESULTS AND CONCLUSION: The time to maximal exhaled hydrogen concentration was (3.25±1.80), (17.8±6.48) and (7.45±1.39) minutes in three ways, respectively, and there were significant differences among the three ways (P < 0.01). The half-life was (7.40±2.09), (141.50±85.01) and (1.20±0.37) minutes, respectively, and there were significant differences among the three ways (P < 0.01). The increment in the area under the curve was (302.17±221.90), (5 234.29±2 681.18), (209.51±     104.49) ppm•min, respectively, and there were significant differences among the three ways (P < 0.01). Hydrogen supply by intraperitoneal injection of saturated hydrogen saline or pure hydrogen both can rapidly increase exhaled hydrogen concentration in rabbits. Intraperitoneal hydrogen can maintain a high exhaled hydrogen concentration for a long time.