Abstract:

BACKGROUND: Several studies have shown that, during confluent culture and monolayer culture, P19 embryonal carcinoma cells can differentiate into myocardial cells induced by 5-azacytidine.
OBJECTIVE: To observe whether P19 embryonal carcinoma cells can differentiate into myocardial cells induced by 5-azacytidine in vitro, as well as the expression of cardiac structural proteins.
METHODS: P19 embryonal carcinoma cells were divided into two groups: experimental group induced by 1 μmol/L 5-azacytidine for 4 days, and control group induced by phosphate buffered saline. Double immunofluorescence staining technique was used to detect the co-expression of Desmin and cardiac troponin T. Western Blot was used to detect the expression of cardiac troponin T.
RESULTS AND CONCLUSION: Co-expression of Desmin and cardiac troponin T was detected in the same part of the same cells in the experimental group, while no expression and co-expression of the two proteins in the control group. And the cardiac troponin T could be detected in the experimental group, while no expression in the control group. The P19 embryonal carcinoma cells under monolayer culture conditions could differentiate into myocardial cells, and express cardiac structure proteins after 5-azacytidine induction in vitro.