Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (11): 1959-1962.doi: 10.3969/j.issn.1673-8225.2012.11.014

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Culture in vitro and identification of human cervical carcinoma cells

Ma Xiu-ping, Cheng Jing-xin, Zhang Yu, Yuan Min   

  1. Department of Gynecology, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi  830000, Xinjiang Uygur Autonomous Region, China
  • Received:2011-10-14 Revised:2011-12-24 Online:2012-03-11 Published:2012-03-11
  • Contact: author: Cheng Jing-xin, Master, Chief physician, Professor, Department of Gynecology, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China 13899899061@139.com
  • About author:Ma Xiu-ping★, Studying for master’s degree, Department of Gynecology, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China mxp8080@163.com
  • Supported by:

    Natural Science Foundation of Xinjiang Uygur Autonomous Region, No. 2010211A31*

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Abstract:

BACKGROUND: There are individual differences in the patients suffered tumor. Therefore the research on the cancer line can not show accurate differences between individuals, while primary cell culture can show more similar characteristics to in vivo state.
OBJECTIVE: To establish a method in vitro for primary culture of cervical carcinoma cells.
METHODS: Cervical cancer cells were obtained from 23 fresh cervical cancer tissues using the trypsin and collagenase type Ⅰdigestion, and their growth curve was detected. Cell surface markers of CK17and CD44 were identified with immunofluorescence staining.
RESULTS AND CONCLUSION: Cervical cancer cells were obtained from 21 of 23 cases, and the cells had the continuous passage and steady proliferation. Immunofluorescence staining showed that the cells positively expressed CK17 and CD44. It is indicated that primary cervical carcinoma cells can be obtained by trypsin and collagenase type Ⅰdigestion.
 

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