Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (9): 1597-1600.doi: 10.3969/j.issn.1673-8225.2012.09.018

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Histopathological changes and rule of migration associated with chondrocytes in acupuncture-induced intervertebral disc degeneration model in rabbits

Wang Shi-jun, Li Chun-de, Sun Hao-lin, Liu Xian-yi, Zhu Tian-yue, Li Wen   

  1. Department of Orthopaedics, First Hospital of Peking University, Beijing  130021, China
  • Received:2011-12-08 Revised:2011-12-23 Online:2012-02-26 Published:2012-02-26
  • Contact: Li Chun-de, Professor, Doctoral supervisor, Department of Orthopaedics, First Hospital of Peking University, Beijing 130021, China lichunde@medmail.com.cn
  • About author:Wang Shi-jun☆, Studying for doctoate, Physician, Department of Orthopaedics, First Hospital of Peking University, Beijing 130021, China doctorwang444@gmail.com
  • Supported by:

    National Natural Science Foundation of China, No. 81071504*; Natural Science Foundation of Beijing, No. 7123231*

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Abstract:

BACKGROUND: The notochordal cells will be replaced by chondrocytes along with the aggravation of degenerative intervertebral disc, but the origin and migration rule of the chondrocytes of the model established by puncturing the annulus fibrosus are still unclear.
OBJECTIVE: To observe the histopathological process of degenerative intervertebral disc model established by puncturing the annulus fibrosus, and to explore the origin and migration rule of the chondrocytes.
METHODS: Twenty-four New Zealand white rabbits were divided into operation group and sham operation group. In the operation group, lumbar discs of L2-3, L3-4, L4-5 and L5-6 of each rabbit were punctured by 16 gauge needle. In the sham operation group, the anterior surfaces of the lumbar discs were exposed without puncture.
RESULTS AND CONCLUSION: The chondrocytes of the nucleus pulposus originated and migrated from the cartilage endplate. At the junction of the nucleus pulposus and the cartilage endplate, the chondrocytes departed from the cartilage endplate to the nucleus pulposus vertically. At the junction of the inner annulus fibrosus and the cartilage endplate, the chondrocytes migrated along the collagen fibers from the cartilage endplate to the shrinked margin of the nucleus pulposus. The non-calcified layer thinned gradually, and the non-calcified layer/calcified layer ratio decreased.
 

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