Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (5): 875-878.doi: 10.3969/j.issn.1673-8225.2012.05.028

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pifithrin-alpha reduces the expression of PUMA protein in the early stage of liver ischemia-reperfusion in rats

Peng Song-lin, Xing Fei, Wang Kai, Zhao Yang, Dai Chao-liu   

  1. Department of Hepatobiliary Surgery, the Affiliated Shengjing Hospital of China Medical University, Shenyang  110004, Liaoning Province, China
  • Received:2011-08-29 Revised:2011-12-06 Online:2012-01-29 Published:2012-01-29
  • Contact: Dai Chao-liu, Doctor, Professor, Chief physician, Department of Hepatobiliary Surgery, the Affiliated Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China daicl@sj-hospital.org
  • About author:Peng Song-lin★, Master, Associate professor, Associate chief physician, Department of Hepatobiliary Surgery, the Affiliated Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China psl514@126.com
  • Supported by:

    Fund of the Affiliated Shengjing Hospital of China Medical University, No. M835*

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Abstract:

BACKGROUND: pifithrin-α is a reversible inhibitor of p53, the effect of pifithrin-α inhibited p53 pathway on hepatic ischemia-reperfusion injury is unclear.
OBJECTIVE: To explore the effect of nuclear transcription factor p53 inhibitor on the expression of PUMA protein after liver ischemia-reperfusion in rats.
METHODS: Ninety-six male Wistar rats were divided into four groups randomly: control group, ischemia-reperfusion group, ischemia-reperfusion+solvent dimethyl sulfoxide (DMSO) group (DMSO group) and ischemia-reperfusion+pifithrin-α group (pifithrin-α group). The 70% liver ischemia model was established. The rats in PFT group were injected with pifithrin-α after 60 minutes, the DMSO group was injected with dimethyl sulfoxide solution and the control group and ischemia-reperfusion group were injected with normal saline in the same dose.
RESULTS AND CONCLUSION: At 1, 3 and 6 hours after ischemia-reperfusion in rat liver, the expression of PUMA protein in liver tissue was obviously, the expression of PUMA protein in pifithrin-α group was inhibited significantly. However, at 24 hours after ischemia-reperfusion, the expression of PUMA protein in pifithrin-α group was higher than that in the other three groups. pifithrin-α can protect liver from ischemia-reperfusion injury through suppressing p53 in the order to decrease the expression of PUMA protein, but pifithrin-α can reduce the expression of PUMA protein only in the early stage after rschemia-reperfusion.

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