Abstract:

BACKGROUND: Studies have reported to promote proliferation, differentiation and regeneration of β-cells is a potential treatment for patients with type 2 diabetes.
OBJECTIVE: To investigate the effects of pancreatotrophin on the proliferation and function of rat islet cells in vitro.
METHODS: The rat islet cells were isolated and purified by collagenase digestion and tissue-culture, followed with the detection of purity and activity. The islet cells were divided into blank control group and experimental group. Normal culture medium was added in the blank control group, pacreatotrophin in different concentrations were put in the experimental groups (100, 200, 300, 400 mg/L respectively). CCK-8 detection was performed to test the proliferous activity after 1, 3 and 5 days. After 5 days of culture, insulin release test was carried out in the condition of low-glucose and high-glucose.
RESULTS AND CONCLUSION: Along with the increase of the pancreatotrophin concentration, the proliferate activity of islet cells was in a dose-dependent manner (P < 0.05), but it demonstrated that there was no obvious time-dependent; In addition to 100 mg/L group and 200 mg/L group, the volume of insulin secretion in 300 mg/L group and 400 mg/L group were significantly higher than that in the blank control group (P < 0.05). It demonstrates that the concentrations of pancreatotrophin in 300 mg/L and 400 mg/L can not only promote cell proliferation, but also can significantly enhance the function of islet cells to secrete insulin