Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (5): 843-846.doi: 10.3969/j.issn.1673-8225.2012.05.020

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Immunomodulatory activity of artesunate to T lymphocytes from the spleens of delayed-type hypersensitivity mice 

Li Qin1,2, Chen Hong2, 3, Bai Shu-fang3, Wei Na3, Zhang Shi3   

  1. 1Department of Immunology, 2Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, 3Department of Pharmacognosy, Medical College of Chinese People’s Armed Police Force, Tianjin  300162, China
  • Received:2011-07-13 Revised:2011-09-22 Online:2012-01-29 Published:2012-01-29
  • Contact: Chen Hong, Doctor, Professor, Doctoral supervisor, Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China; Department of Pharmacognosy, Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China chenhongtian06@yahoo.com.cn
  • About author:Li Qin☆, Doctor, Lecturer, Department of Immunology, Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China; Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China Tanli20042001@yahoo.com.cn
  • Supported by:

    the Postdoctoral Science Foundation of China (General Program), No. 20090461498*; Applied Basis and Frontier Technique Research Plan of Tianjin, No. 11JCYBJC14600*; China Postdoctoral Science Foundation, No. 201104796*

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Abstract:

BACKGROUND: Artesunate is a derivant of high efficiency and low toxicity from artemisinin, which has been shown to affect immune response. However, the underlying mechanism remains elusive.
OBJECTIVE: To investigate the immunoregulatory mechanism of artesunate to T lymphocytes from the spleens of delayed-type hypersensitivity mice.
METHODS: The effects of artesunate on the ear swelling and spleen index were measured in delayed-type hypersensitivity mice. The transformation of spleen lymphocytes was used to detect the proliferation of T cells. Western blot assay was applied to investigate the p38 mitogen-activated protein kinase (MAPK) activation. The proliferation of T lymphocytes was detected by T lymphocyte transformation test. MAPK activity was detected by western blot method.
RESULTS AND CONCLUSION: Topical application of artesunate could suppress the increase in ear thickness, spleen index and ConA-induced T lymphocytes proliferation significantly. Furthermore, artesunate was observed to decrease the phosphorylation of p38 MAPK. These demonstrated that artesunate had an anti-delayed-type hypersensitivity effect through modulating p38MAPK pathways, which might be beneficial for immune-related diseases.

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