Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (50): 9385-9388.doi: 10.3969/j.issn.1673-8225.2011.50.019

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Establishment of a radial bone critical defect model in a rabbit

Zhou Fang1, Li Jing1, Yu Lei2, Liao Hua2, Ouyang Jun2   

  1. 1Southern Medical University, Guangzhou  510515, Guangdong Province, China
    2Department of Human Anatomy, Southern Medical University, Guangdong Provincial Key Laboratory of Tissue Construction and Inspection, Guangzhou 510515, Guangdong Province, China
  • Received:2011-05-27 Revised:2011-07-12 Online:2011-12-10 Published:2011-12-10
  • Contact: Liao Hua, Doctor, Professor, Department of Human Anatomy, Southern Medical University, Guangdong Provincial Key Laboratory of Tissue Construction and Inspection, Guangzhou 510515, Guangdong Province, China Correspondence to: Ouyang Jun, Doctor, Professor, Department of Human Anatomy, Southern Medical University, Guangdong Provincial Key Laboratory of Tissue Construction and Inspection, Guangzhou 510515, Guangdong Province, China jouyang@126.com
  • About author:Zhou Fang☆, Studying for doctorate, Southern Medical University, Guangzhou 510515, Guangdong Province, China Li Jing, Studying for doctorate, Southern Medical University, Guangzhou 510515, Guangdong Province, China Liyu12399@126.com Zhou Fang and Li Jing contributed equally to this paper.
  • Supported by:

    the National High Technology Research and Development Program of China (863 Project), No. 2006AA02A118*

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Abstract:

BACKGROUND: Nonunion caused by various reasons of fractures and bone defects has been a major problem of clinical orthopedic rehabilitation. With the rapid development of tissue engineering, product quality testing needs standard bone defect model. However, the length of bone critical-size defects has not been ascertained.
OBJECTIVE: To establish rabbit radial bone defect models and to determine the length of rabbit radial bone critical-size defects.
METHODS: Eighteen male New Zealand white rabbits (2.0-2.5 kg) were randomly assigned into 6 groups and then 12, 13, 14, 15, 16 and 17 mm segmental defects were created in the middle part of radius on both sides, respectively. The incisions were sutured and bandaged, but not fixed.
RESULTS AND CONCLUSION: According to examination with naked eyes and X-ray, some of the segmental defects with the length of 12, 13, 14, 15 and 16 mm were completely repaired. But none of the defects with the length of 17 mm was repaired. Histological examination showed that there was trabecular bone formation, bone matrix, medullary cavity recanalization, revascularization and osteoblasts in the segmental defects with the length of 12, 13, 14, 15 and 16 mm. However, there were osteoblasts, osteoclasts in the 17 mm defects, but no medullary cavity recanalization or revascularization. Therefore, the length of bone critical-size defect in rabbit radius was 17 mm.

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