Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (50): 9331-9334.doi: 10.3969/j.issn.1673-8225.2011.50.006

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Culture of human bronchial epithelial cells in vitro

Wu Qi-biao1, Lu Jin-fu2, Yin Yao-ting1, Cao Shi-hong3   

  1. 1Faculty of Chinese Medicine, Macau University of Science and Technology, Macau  999078, China
    2School of Pharmacology, Nanjing University of Chinese Medicine, Nanjing  210029, Jiangsu Province, China
    3Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China
  • Received:2011-05-19 Revised:2011-08-29 Online:2011-12-10 Published:2011-12-10
  • Contact: Lu Jin-fu, Doctor, Associate professor, School of Pharmacology, Nanjing University of Chinese Medicine, Nanjing 210009, Jiangsu Province, China ljf_909@yahoo.com.cn
  • About author:Wu Qi-biao☆, Doctor, Assistant professor, Master’s supervisor, Faculty of Chinese Medicine, Macau University of Science and Technology, Macau 999078, China qbwu@must.edu.mo
  • Supported by:

    the Science and Technology Development Fund, Macao, China, No. 003/2005/A*, 027/2007/A2*

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Abstract:

BACKGROUND: Culture of human bronchial epithelial cells has been increasingly applied in the research of respiratory diseases.
OBJECTIVE: To explore a feasible in vitro culture method for human bronchial epithelial cell line supplied by American Type Culture Collection.
METHODS: Different culture conditions of human bronchial epithelial cells were tested with trials and errors. A feasible method with DMEM/F12 containing 20% fetal bovine serum was finalized.
RESULTS AND CONCLUSION: The cultured human bronchial epithelial cells were flat, polygonal, and displayed a cobblestone-like distribution when overgrown. Immunohistochemical staining results showed an expression of epithelial marker keratin in cultured cells. The expression of nuclear factor κB, tumor necrosis factor α and interleukin 8 was increased significantly in cultured cells exposed to sputum supernatant from patients with bronchiectasis. These findings demonstrated that the cultured human bronchial epithelial cells were obtained successfully. Therefore, there is no need to stick to the culture condition recommended by American Type Culture Collection. The modified culture method is simple, feasible and valuable.

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