Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (41): 7723-7725.doi: 10.3969/j.issn.1673-8225.2011.41.030

Previous Articles     Next Articles

Construction and identification of lentiviral vector of RNA interference of prolyl-4 hydroxylase-2 gene

Ma Fang-fang1, Wang Hou-zhao1, Shen Xiao li2   

  1. 1Department of Laboratory Medicine, the 174 Hospital of Chinese PLA, Xiamen  361003, Fujian Province, China
    2Key Laboratory of Cardiovascular Disease Fujian Provincial Hospital Fuzhou  350001, Fujian Province, China
  • Received:2011-03-04 Revised:2010-03-10 Online:2011-10-08 Published:2011-10-08
  • About author:Ma Fang-fang★, Master, Laboratorian, Department of Laboratory Medicine, the 174 Hospital of Chinese PLA, Xiamen 361003, Fujian Province, China 13178355953@163. com

PDF

321

Abstract:

BACKGROUND: Lentiviral vector can stably mediate gene silencing with high transfection efficiency.
OBJECTIVE: To construct and identify a lentiviral vector of RNA interference (RNAi) of prolyl-4 hydroxylase-2 (P4HA2) gene.
METHODS: The effective sequence of siRNA targeting P4HA2 gene was confirmed in our previous study. The complementary DNA containing both sense and antisense Oligo DNA of the targeting sequence was designed, synthesized and cloned into the pGCSIL-GFP vector to construct a lentiviral vector which expressed short hairpin RNA (shRNA), and it was identified by PCR and DNA sequencing.
RESULTS AND CONCLUSION: PCR identification and DNA sequencing demonstrated that insertion of oligonucleotide of the lentivirus RNAi vector containing P4HA2 shRNA was right. Results suggested that the lentivirus RNAi vector of P4HA2 was constructed successfully.

CLC Number: