Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (41): 7715-7718.doi: 10.3969/j.issn.1673-8225.2011.41.028

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Retinal erythropoietin expression in embryonic and postnatal rats

Meng Xu-xia, Zhang Yan, Niu Ying-yun   

  1. Department of Ophthalmology, Affiliated Hospital of Qingdao University Medical School, Qingdao  266003, Shandong Province, China
  • Received:2011-04-03 Revised:2011-05-24 Online:2011-10-08 Published:2011-10-08
  • About author:Meng Xu-xia★, Master, Chief physician, Department of Ophthalmology, Affiliated Hospital of Qingdao University Medical School, Qingdao 266003, Shandong Province, China mengxuxia@163. com
  • Supported by:

    a grant from Qingdao Science and Technology Bureau, No. 09-1-1-18-nsh*

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Abstract:

BACKGROUND: Erythropoietin (EPO) is downstream target gene of hypoxia inducible factor-1 and regulated by hypoxia inducible factor-1.
OBJECTIVE: To investigate EPO expression during embryonic development of rat retina and postnatal early development and explore the role of EPO in retinal development.　
METHODS: Five embryo of 12, 16, 20 days and five adult Wistar rats were sacrificed. Their eyeballs were harvested and retina was separated. EPO protein and mRNA expression was detected by immunohistochemistry and RT-PCR, respectively.　
RESULTS AND CONCLUSION: EPO expression was detected in cytoplasm and nuclei of neuroepithelium of retina and pigment epithelial cells during embryonic development of rat retina. The expression was gradually decreased. EPO expression was the weakest in adult rat retina. Results showed that EPO expression exhibited spatial changes during embryonic development of rat retina, which may correlate closely to the embryonic development of rat retina.

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