Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (41): 7685-7688.doi: 10.3969/j.issn.1673-8225.2011.41.021

Previous Articles     Next Articles

Effects of treadmill running on hippocampal neurogenesis and mRNA expression in vascular endothelial growth factor in a rat model of ischemia and reperfusion injury

Yang Ruo-yu1, Lou Shu-jie2, Chen Xiu-en3   

  1. 1Research Station of Sport Science of Shanghai Huangpu District, Shanghai 200010, China
    2School of Sport Science, Shanghai University of Sport, Shanghai  200438, China
    3Department of Rehabilitation Medicine, Shanghai Huadong Hospital, Shanghai  200040, China
  • Received:2011-04-06 Revised:2011-07-12 Online:2011-10-08 Published:2011-10-08
  • About author:Yang Ruo-yu★, Assistant Researcher, Research Station of Sport Science of Shanghai Huangpu District, Shanghai 200010, China ryyang999@ hotmail.com

PDF

325

Abstract:

BACKGROUND: Several studies have demonstrated that treadmill running can promote hippocampal neurogenesis in healthy rats.
OBJECTIVE: To investigate the effects of treadmill running on hippocampal neurogenesis and mRNA expression in vascular endothelial growth factors (VEGF) in a rat model of ischemia and reperfusion injury.
METHODS: The method of reversible middle cerebral artery occlusion (MCAO) was used to obtain rat models of ischemia and reperfusion injury. MCAO rat models were randomly divided into exercise group and control group. A sham-operated group was used. Rats in the control and sham-operated groups were fed without exercise. The exercise group rats were subjected to 7-day treadmill running. The exercise and control group rats were intraperitoneally injected with 5-bromodeoxyuridine (BrdU) solution.
RESULTS AND CONCLUSION: Immunohistochemical staining results showed that BrdU-positive cells in the bilateral hippocampus and dentate gyrus were significantly more in the exercise group than in the control group (P < 0.01). Real time quantitative PCR results showed that mRNA expression in VEGF was significantly greater in the exercise group than in the control and sham-operated groups (P < 0.05). The results suggest that appropriate treadmill running can promote hippocampal neurogenesis and further enhance the mRNA expression in hippocampal VEGF in ischemia and reperfusion adult rats.

CLC Number: