Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (41): 7681-7684.doi: 10.3969/j.issn.1673-8225.2011.41.020

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Effects of osthole on fibroblast proliferation and transforming growth factor beta 1 expression in hypertrophic scar tissue

Hou Xiao-hua1, Chen Hong2, Cao Bo2   

  1. 1Department of Burn and Plastic Surgery, Affiliated Hospital of Medical College of Chinese People's Armed Police Forces, Tianjin  300162, China
    2Department of Pharmacognosy, Medical College of Chinese People’s Armed Police Forces, Tianjin  300162, China
  • Received:2011-03-18 Revised:2011-03-28 Online:2011-10-08 Published:2011-10-08
  • About author:Hou Xiao-hua★, Master, Attending physician, Department of Burn and Plastic Surgery, Affiliated Hospital of Medical College of Chinese People’s Armed Police Forces, Tianjin 300162, China houxiaohua73@sina.com

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Abstract:

BACKGROUND: Osthole exhibits inhibitory effects on human fibroblast proliferation and transforming growth factor beta 1 (TGF-β1) expression in hypertrophic scar tissue, but the precise mechanisms remains poorly understood.
OBJECTIVE: To investigate the effects of osthole on human fibroblast proliferation and TGF-β1 expression in hypertrophic scar tissue.
METHODS: Human hypertrophic scar fibroblasts cells were cultured in vitro and then treated by osthole at different concentrations. The growth inhibitory effects were observed by MTT assay and cell growth curve. The expression of TGF-β1 was detected by immunohistochemistry.
RESULTS AND CONCLUSION: Osthole could obviously inhibit the growth of human hypertrophic scar fibroblasts. MTT assay showed that osthole IC50 value toward hypertrophic scar fibroblasts was 15.2±2.0 μmol/L. Furthermore, the results of cell growth curve matched with the above results. Immunohistochemistry results showed that osthole could obviously inhibit TGF-β1 expression in the fibroblasts derived from hypertrophic scar tissue compared with the control group (P < 0.05). These findings suggest that osthole strongly inhibits the growth of human hypertrophic scar fibroblasts and decreases the expression of TGF-β1.

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