Abstract:

BACKGROUND: Biliary scar formation results in destroyed balance between cell proliferation and apoptosis, in particular, fibroblasts greatly proliferate and excessively secrete collagen. 
OBJECTIVE: To compare nuclear factor-kappa B (NF-κB) and cellular Fas-associated death domain-like interleukin-1 beta-convening enzyme (FLICE) inhibitory protein expression in fibroblasts from normal bile duct and biliary scar tissue of Diannan small-eared pigs.
METHODS: Eight Diannan small-eared pigs were randomly divided into model group and normal control group. In the model group, biliary scar tissue-derived fibroblasts were harvested by establishing bile duct injury models. In the normal control group, normal bile duct fibroblasts were harvested.
RESULTS AND CONCLUSION: Compared with normal bile duct-derived fibroblasts, NF-κB and FLICE inhibitory protein expression was greater in the biliary scar tissue-derived fibroblast. These findings suggest that after biliary duct injury, NF-κB expression was increased and FLICE inhibitory protein was overexpressed, which leads to reduction in bile duct fibroblasts and finally results in biliary scar formation.