Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (41): 7669-7672.doi: 10.3969/j.issn.1673-8225.2011.41.017

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Detection of autocrine transforming growth factor-beta 1 concentration in the primary fibroblasts and fibrosarcoma cells and effects on cell proliferation

Liu Ping, Li Ping, Peng Yan, Yang Nan, Zhou Yuan-guo   

  1. State Key Laboratory of Trauma, Burns and Combined Injury, Center of Molecular Biology, Research Institute of Field Surgery, Daping Hospital, Third Military Medical University of Chinese PLA, Chongqing 400042, China
  • Received:2011-03-18 Revised:2011-05-04 Online:2011-10-08 Published:2011-10-08
  • Contact: Li Ping, Doctor, Assistant researcher, State Key Laboratory of Trauma, Burns and Combined Injury, Center of Molecular Biology, Research Institute of Field Surgery, Daping Hospital, Third Military Medical University of Chinese PLA, Chongqing 400042, China ping__ping0074@ sina.com
  • About author:Liu Ping, Senior experimentalist, State Key Laboratory of Trauma, Burns and Combined Injury, Center of Molecular Biology, Research Institute of Field Surgery, Daping Hospital, Third Military Medical University of Chinese PLA, Chongqing 400042, China liuping731@yahoo.com.cn
  • Supported by:

    Innovation Youth Foundation of Third Military Medical University of Chinese PLA*; the National Natural Science Foundation of China, No. 30801195*

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Abstract:

BACKGROUND: Although tremendous strides have been made in clarifying the different regulation mechanisms of cell proliferation on normal cells and tumor cells, until now, it is still not clear. Exogenous stimulation of transforming growth factor-β1 (TGF-β1) is often used in vitro experiments to observe the changes in cell proliferation and the effects of autocrine TGF-β1 itself are easily ignored.
OBJECTIVE: To detect the concentration of autocrine TGF-β1and cell proliferation of the primary fibroblasts (Fb) and fibrosarcoma cells (L929), and to investigate the effects of the autocrine TGF-β1 on cell proliferation.
METHODS: The TGF-β1 levels in cells and supernatants of Fb and L929 cells were detected by ELISA test kit. The proliferation of Fb and L929 cells were detected by MTT assay.
RESULTS: TGF-β1 concentration in L929 cells increased with prolonged incubation time, whereas TGF-β1 concentration in Fb cells decreased with prolonged incubation time. TGF-β1 concentration in supernatant of Fb and L929 cells increased with prolonged incubation time, but TGF-β1 concentration in supernatant of L929 cells was significantly higher than that of Fb cells   (P < 0.01). The cell proliferation curves both in Fb and L929 cells were significantly increased within 72 hours (P < 0.01). Compared with the same time, the cell proliferation of L929 cells was significantly higher than that of Fb cells. The autocrine TGF-β1 of the Fb and L929 cells can promote cell proliferation, and different levels of autocrine TGF-β1 maybe the important reason for different reactions of Fb and L929 cells stimulated by exogenous TGF-β1.

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