Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (41): 7657-7660.doi: 10.3969/j.issn.1673-8225.2011.41.014

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Effects of compound panax notoginseng on proliferating cell nuclear antigen expression in liver tissue of rats exposed to formaldehyde vapor

Wang Jian-wei1, Liu Sheng-yong1, Lei Xiu-bing2, Wang Zhen-yu3, Cheng Jia-mao4, Chen Feng1, Yue Bing1, Wang Ke1, Tian Long1, Zhang Wei-guang1   

  1. 1Department of Anatomy, Histology and Embryology, Peking University Health Science Center, Beijing  100191, China
    2Department of Medicine, Panzhihua University, Panzhihua   617000, Sichuan Province, China
    3Hospital of Peking University Health Science Center, Beijing  100191, China
    4School of Basic Medical Sciences, Dali University, Dali   671000, Yunnan Province, China
  • Received:2011-05-06 Revised:2011-08-16 Online:2011-10-08 Published:2011-10-08
  • Contact: Zhang Wei-guang, Master, Associate professor, Department of Anatomy, Histology and Embryology, Peking University Health Science Center, Beijing 100191, China zhangwg@bjmu.edu.cn
  • About author:Wang Jian-wei★, Studying for master’s degree, Department of Anatomy, Histology and Embryology, Peking University Health Science Center, Beijing 100191, China wjw@bjmu.edu.cn
  • Supported by:

    the National Science Foundation for Fostering Talents in Basic Research of the National Natural Science Foundation of China, No. J0630853/0108*

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Abstract:

BACKGROUND: Formaldehyde is a commonly used chemical substance which can cause serious damage to the body as a toxicant, and there is lack of effective control methods.
OBJECTIVE: To explore the curative effect of compound panax notoginseng (CPN) on the proliferation of hepatic cells of Sprague-Dawley rats exposed to formaldehyde vapor.
METHODS: Eighteen Sprague-Dawley male rats were randomly divided into three groups: control, formaldehyde and CPN. Nothing was done to the control group rats. The formaldehyde group rats were exposed to formaldehyde vapor in static toxification chambers, 8 hours/day, 5 days/week, for a total of 8 weeks. The CPN group rats were treated as the same the formaldehyde group, with exception of intragastric administration of CPN once a day since the 5th week.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining results showed that the hepatic lobule morphology of the formaldehyde group significantly disordered at the 8th week, which suggested an obvious proliferation state, and the hepatic sinusoid was narrower compared with the control group; however, the morphological structure of liver tissue in the CPN group was greatly recovered. Immunohistochemical results showed that the expression of proliferating cell nuclear antigen (PCNA) was significantly increased at the 8th week in the formaldehyde group than in the control group (P < 0.01), but the expression was significantly decreased in the CPN group than in the formaldehyde group (P < 0.01). These findings suggest that CPN can decrease the expression of PCNA which was induced by formaldehyde and effectively alleviate formaldehyde-induced hepatic cell proliferation, therefore exhibiting beneficial effects on the liver of SD rats exposed to formaldehyde vapor.

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