Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (41): 7653-7656.doi: 10.3969/j.issn.1673-8225.2011.41.013

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Vascular endothelial growth factor and nuclear factor-kappa B1 expression in a nude mouse model of MG63 and MG63/ADR osteosarcoma

Sun Jiao1, Zhang Chun-lin2, Wu Xiao-san3, Zhao Shi-chang2, Zhao Yao2, Zeng Bing-fang2   

  1. 1Department of Orthopedics, Shanghai Sixth People's Hospital, School of Medicine， Soochow University, Shanghai  200233, China
    2Department of Orthopedics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai  200233, China
    3Department of Orthopedics, Liuan People's Hospital, Liuan  237005, Anhui Province, China
  • Received:2011-04-01 Revised:2011-06-06 Online:2011-10-08 Published:2011-10-08
  • Contact: Zhang Chun-lin, Associate chief physician, Department of Orthopedics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200233, China shzhangchunlin@ gmail.com
  • About author:Sun Jiao★, Studying for master’s degree, Physician, Department of Orthopedics, Shanghai Sixth People's Hospital, School of Medicine, Soochow University, Shanghai 200233, China sun8516@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30700850*

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Abstract:

BACKGROUND: Studies concerning sarcoma drug resistance found that vascular endothelial growth factor (VEGF) and nuclear factor-kappa B1 (NF-κB1) may be closely related to drug resistance, but few studies are involved in osteosarcoma. Establishment of animal models and gene expression of VEGF and NF-κB1 may reveal the association between genes and drug resistance in osteosarcoma.
OBJECTIVE: To observe gene expression differences of VEGF and NF-κB1 in a nude mouse model of MG63 and MG63/ADR osteosarcoma.
METHODS: An adriamycin-resistant human osteosarcoma cell subline (MG63/ADR) was established in vitro using gradually increased concentration of adriamycin (ADM) in culture. BALB/C nude mice were divided into MG63 and MG63/ADR groups with 10 mice in each group. The animal model of osteosarcoma xenografts was constructed by subcutaneous inoculation of osteosarcoma MG63 and MG63/ADR cells respectively. Two group mice were killed at 6 weeks and gene expression differences of VEGF and NF-κB1 in the tumor formation of two groups were detected using immunohistochemistry and RT-PCR method.
RESULTS AND CONCLUSION: Both VEGF and NF-κB1 expression in MG63/ADR group was significantly higher compared with the MG63 group. The upregulation of VEGF and NF-κB1 may be an important reason of drug resistance in osteosarcoma, providing an experimental basis for exploring the molecular mechanism of drug resistance in osteosarcoma and finding more effective targets of osteosarcoma treatment.

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