Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (41): 7619-7622.doi: 10.3969/j.issn.1673-8225.2011.41.005

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Effect of geniposide on proliferation of rat chondrocytes in vitro

Tan Yu-lin1, Bao Tong-zhu2, Liu Qin2, Han Yu3, Wang Yan-lin3, Zhou Na-xin2, Chen Ken2, Yan Fei2   

  1. 1The First College of Clinical Medical Science, China Three Gorges University, Yichang 443003, Hubei Province, China
    2Yichang Central People’s Hospital, Yichang 443003, Hubei Province, China
    3Institute of Molecular Biology, Three Gorges University, Yichang 443003, Hubei Province, China
  • Received:2011-03-05 Revised:2011-03-19 Online:2011-10-08 Published:2011-10-08
  • Contact: Bao Tong-zhu, Master, Chief physician, Yichang Central People’s Hospital, Yichang 443003, Hubei Province, China baotongzhu1963@ 163.com
  • About author:Tan Yu-lin★, Studying for master’s degree, the First College of Clinical Medical Science, China Three Gorges University, Yichang 443003, Hubei Province, China yltan2008@126.com
  • Supported by:

    the Natural Science Foundation of Hubei Province, No. 2010CDB10701*

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Abstract:

BACKGROUND: Studies have shown that geniposide can ameliorate the treatment of ligament injuries by proliferating ligament fibroblasts and promoting the synthesis of collagen.
OBJECTIVE: To investigate the effect of geniposide on the proliferation of rat chondrocytes in vitro.
METHODS: The rat articular chondrocytes were isolated by enzyme digestion and sub-cultivated in serial. The chondrocytes of passage 4 were interfered with 0, 25, 50, 100 mmol/L geniposide. Chonddrocyte proliferation was detected using methyl thiazolyl tetrazolium and flow cytometry.
RESULTS AND CONCLUSION: The phenotype of chondrocyte was changed from original polygonal shape to fusiform with the increased passage. The proliferation of chondrocytes in the 25, 50,100 mmol/L geniposide groups was respectively higher than that in the control group (P < 0.01). Geniposide can stimulate the proliferation of chondrocytes.

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