Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (41): 7611-7614.doi: 10.3969/j.issn.1673-8225.2011.41.003

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Insulin-like growth factor 1 and transformation growth factor beta 2 promote engineered cartilage formation in vitro

Chen Ning-jie1, 2, Zhang Wei1, Liang Xiao-qin2, Yang Biao-bing2   

  1. 1Plastic Surgery Research Institution, Weifang Medical College, Weifang 261042, Shandong Province, China
    2Department of Aesthetic Plastic Surgery, Plastic Surgery Hospital of Weifang Medical College, Weifang  261042, Shandong Province, China
  • Received:2011-03-01 Revised:2011-05-01 Online:2011-10-08 Published:2011-10-08
  • Contact: Yang Biao-bing, Chief physician, Professor, Master’s supervisor, Department of Aesthetic Plastic Surgery, Plastic Surgery Hospital of Weifang Medical College, Weifang 261042, Shandong Province, China ybiaobing@163.com
  • About author:Chen Ning-jie★, Master, Pastic Surgery Research Institution, Weifang Medical College, Weifang 261042, Shandong Province, China; Department of Aesthetic Plastic Surgery, Plastic Surgery Hospital of Weifang Medical College, Weifang 261042, Shandong Province, China cnfly101942@163. com
  • Supported by:

    Science and Technology Development Program of Higher Education of Shandong Province, No.J09LF20*

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Abstract:

BACKGROUND: Cartilage tissue has poor regenerative capacity. Cartilage tissue engineering can repair damaged cartilage tissue using fewer cells, scaffold materials and cytokines. 
OBJECTIVE: To investigate the effects of insulin-like growth factor (IGF-1) combined with transformation growth factor beta 2 (TGF-β2) on engineered cartilage formation.
METHODS: Human chondrocytes were isolated by enzyme digestion method and then were inoculated onto calcium alginate bead scaffold for culture in three-dimensional circumstance after addition of 200 μg/L IGF-1 and (or) 1 μg/L TGF-β2. At 3, 5, 7, 9, 11, and 13 days of culture, chondrocyte proliferation was tested by cell counting. After 2 weeks of culture, the fresh tissues were assessed by morphological observation, alcian blue-periodic acid sthiff staining, and anti typeⅡcollagen immunohistochemicel staining.
RESULTS AND CONCLUSION: IGF-1 and TGF-β2 could stimulate chondrocytes to proliferate and excrete chondral matrix. IGF-1 mainly promoted the proliferation of chondrocytes, while TGF-β2 mainly promoted the formation of cartilage-related matrix. IGF-1 combined with TGF-β2 has synergistic effects on promoting engineered cartilage formation.

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